Mass spec headers for Boron and Calcium geochemistry of mussel larvae bulk material, 2014-2015 (OA_Proxies project)
Project
Program
| Contributors | Affiliation | Role |
|---|---|---|
| Herrmann, Achim D. | Louisiana State University (LSU-CSI) | Principal Investigator |
| Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Abstract
These data are the headers from the mass spectrometer runs of mussel larvae shells, conducted on 4 Jan. 2014 and 1 June 2015.
The concentrations of B and Ca in each mussel were analyzed using a laser ablation inductively coupled plasma mass spectrometer (LA-ICPMS; Cetac LSX-213; 213 nm laser coupled to an iCap Qc ICPMS) located in the Department of Geology and Geophysics at Louisiana State University. Boron isotopes of bulk mussel larvae and additional B/Ca ratios were measured in the Secondary Ion Mass Spectrometry Labs at Arizona State University using a Cameca IMS 3F.
LA-ICPMS data can be evaluated using Iolite. SIMS data was evaluated using an instrument fractionation factor as established from Bahamian carbonates.
BCO-DMO Data Processing:
In order to serve on the BCO-DMO system, these changes were made to the originally submitted files:
- headers were split off from data
- separated each pair of meta/value into single record
- sorted files by time_start
- added columns for date, run, sample, time_start, meta, and value
| File |
|---|
mass_spec_headers.csv (Comma Separated Values (.csv), 693.79 KB) MD5:89d55b92e728a91dafbcf9abf9586b5c Primary data file for dataset ID 518621 |
| File |
|---|
SIMS results table: Secondary Ion Mass Spectrometry filename: Hermann_SIMS_data_3July2014.pdf (Portable Document Format (.pdf), 57.29 KB) MD5:4504cb9efc0d344f06104ae396335c35 |
| Parameter | Description | Units |
| date | date of analysis | yyyymmdd |
| run | mass spec run id | unitless |
| sample | sample identification | unitless |
| time_start | time at start of mass spec run | HH:MM:SS |
| meta | mass spectrometer parameter | unitless |
| value | value of parameter | various |
| Dataset-specific Instrument Name | ICP Mass Spec |
| Generic Instrument Name | Inductively Coupled Plasma Mass Spectrometer |
| Dataset-specific Description | LA-ICPMS; Cetac LSX-213; 213 nm laser coupled to an iCap Qc ICPMS located in the Department of Geology and Geophysics at Louisiana State University. |
| Generic Instrument Description | An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. |
| Dataset-specific Instrument Name | Mass Spec |
| Generic Instrument Name | Mass Spectrometer |
| Dataset-specific Description | Cameca IMS 3F located at the Secondary Ion Mass Spectrometry Labs at Arizona State University |
| Generic Instrument Description | General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. |
Herrmann_lab
| Website | |
| Platform | lab LSU |
| Start Date | 2014-01-01 |
| End Date | 2015-06-30 |
| Description | Elemental analyses of organisms |
Development of geochemical proxies to evaluate larval pH-exposure history (OA_Proxies)
This research is funded as part of NSF CRI Ocean Acidification Category 2. The investigators will develop a new interdisciplinary partnership between connectivity ecology (Levin at SIO), metal isotope geochemistry (Anbar and Gordon at ASU), and paleoclimatology (Herrmann at ASU/LSU) to identify new proxies for ocean acidification that can be used to assess pH exposures in living organisms and, potentially to interpret the geologic record. The investigators hypothesize that the isotopic composition of larval calcium carbonates reflects changes in seawater chemistry driven by ocean acidification and, in some instances, with associated decline in oxygen levels. The large extent to which these two parameters vary in concert in the modern and past ocean (and thus have joint influence), and the extent to which they may be uncoupled by anthropogenic CO2 inputs, merits considerable attention. Thus, the integration of pH and oxygen in proxy development would be an important advance.
The focus of this project is on proxy development to determine pH exposure history for living organisms in their larval state, and will center on calcium, boron, and uranium isotopes as well as multi-elemental fingerprints. For this project, the investigators will target open coast, front bay and backbay mytilid mussel species, each living naturally under a different pH regime, and statoliths of encapsulated market squid larvae from the open shelf. Larvae with known pH, oxygen and temperature exposure histories will be obtained from (1) laboratory larval rearing experiments that manipulate pH and oxygen and (2) in situ out planting of lab-spawned larvae in larval homes onto existing moorings where pH, T and oxygen are being monitored. Analyses will employ SIMS (for del 11B), multicollector (for del 44Ca, del 238 U), and laser ablation ICP-MS (targeting B, Cu, U, Pb, Mo, and a suite of additional pH- and redox-sensitive trace elements). Multivariate statistical tools will define ability to detect pH-induced signatures and to determine species or taxon-specific vital effects. The investigators are exploring proxies for invertebrate larvae that are untested in the context of acidification geochemistry. Targeting larvae is critical as many marine organisms produce larval carbonate structures and these stages may be most affected by ocean acidification. The retention of larval shell and statoliths after recruitment may ultimately allow us to test the importance of larval pH and O2 exposure to survival and population persistence. An ability to assess past exposures through geochemical proxies will provide information about relative pH tolerances and ecosystem-level change in response to changes in the ocean's carbonate chemistry.
NOTE: A series of laboratory experiments were run in which Mytilus spp. larvae (Mytilus californianus and Mytilus galloprovincialis) and Doryteuthis opalescens (market squid) embryos were reared under controlled temperature, pH and oxygen conditions. Experimental conditions are given in Table 1 (for mussel larvae) and Table 2 (for squid embryos). Geochemistry data in the form of Metal:Ca ratios for mussels has been uploaded to BCO-DMO as "Mussel shell trace element ratios" and squid statolith geochemistry data are available on request.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
| Funding Source | Award |
|---|---|
| NSF Division of Ocean Sciences (NSF OCE) |
[ table of contents | back to top ]