Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Quantum yield of photosynthesis (Fv/Fm) and lifetime fluorescence data from the MV1405 cruise (IrnBru).

[Lifetime fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. This decay in fluorescence can be measured over a wide time range: from picoseconds to milliseconds and beyond. - DMO] The average lifetime of these data are found in the TAV column.

A custom-built Fast Repetition Rate Fluorometer and Lifetime Fluorometer measured surface water continuously using the ship's underway water system. Background fluorescence was measured daily using 0.2 um filtered seawater and manually subtracted from sample data. 

For a complete description of data processing see Kuzminov and Gorbunov (2015)

BCO-DMO Processing Notes:
-filled in all blank cells with nd according to description in metadata
-changed column names to meet BCO-DMO naming standards
-removed header from file because repeated at toplevel
-separated the dateTime GMT column into two columns, date_gmt and time_gmt
-added a cruise_id and ISO_DateTime_UTC column

Description from NSF award abstract:
Diatoms, unicellular, eukaryotic photoautotrophs, are among the most ecologically successful and functionally diverse organisms in the ocean. In addition to contributing one-fifth of total global primary productivity, diatoms are also the largest group of silicifying organisms in the ocean. Thus, diatoms form a critical link between the carbon and silicon (Si) cycles. The goal of this project is to understand the molecular regulation of silicification processes in natural diatom populations to better understand the processes controlling diatom productivity in the sea. Through culture studies and two research cruises, this research will couple classical measurements of silicon uptake and silica production with molecular and biochemical analyses of Silicification-Related Gene (SiRG) and protein expression. The proposed cruise track off the West Coast of the US will target gradients in Si and iron (Fe) concentrations with the following goals: 1) Characterize the expression pattern of SiRGs, 2) Correlate SiRG expression patterns to Si concentrations, silicon uptake kinetics, and silica production rates, 3) Develop a method to normalize uptake kinetics and silica production to SiRG expression levels as a more accurate measure of diatom activity and growth, 4) Characterize the diel periodicity of silica production and SiRG expression.

It is estimated that diatoms process 240 Teramoles of biogenic silica each year and that each molecule of silicon is cycled through a diatom 39 times before being exported to the deep ocean. Decades of oceanographic and field research have provided detailed insight into the dynamics of silicon uptake and silica production in natural populations, but a molecular understanding of the factors that influence silicification processes is required for further understanding the regulation of silicon and carbon fluxes in the ocean. Characterizing the genetic potential for silicification will provide new information on the factors that regulate the distribution of diatoms and influence in situ rates of silicon uptake and silica production. This research is expected to provide significant information about the molecular regulation of silicification in natural populations and the physiological basis of Si limitation in the sea.