Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)

Website: https://www.bco-dmo.org/dataset/732438
Data Type: experimental
Version: 1
Version Date: 2018-03-22

Project
» Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program
» Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)
ContributorsAffiliationRole
Kubler, Janet E.California State University Northridge (CSUN)Principal Investigator
Dudgeon, SteveCalifornia State University Northridge (CSUN)Co-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Abstract
This dataset reports the chlorophyll a concentration per unit biomass of Ulva lactuca grown in closed culture pots at varying pCO2 levels.


Coverage

Spatial Extent: N:34 E:-118 S:33 W:-119
Temporal Extent: 2015-05-11 - 2015-07-27

Dataset Description

This dataset reports the chlorophyll a concentration per unit biomass of Ulva lactuca grown in closed culture pots at varying pCO2 levels.

Related Datasets:
Ulva: Carbonate chemistry pCO2: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: CHN and stable isotopes: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Ulva: Growth: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
Ulva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)


Methods & Sampling

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining nitrogen, oxygen and carbon dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being nitrogen.

Chlorophyll a was extracted from Ulva tissue following the procedure described in Duncan and Harrison (1982). Chlorophyll a concentrations were measured using a Shimadzu UV-2450 UV-visible spectrophotometer.


Data Processing Description

BCO-DMO Processing Notes:
- added a conventional header with dataset name and description, PI names, version date
- modified parameter names to conform with BCO-DMO naming conventions


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Data Files

File
Chl_a_Ulva.csv
(Comma Separated Values (.csv), 6.06 KB)
MD5:13302458522a7c50790cb4247dfd48f0
Primary data file for dataset ID 732438

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Related Publications

Duncan, M. J., & Harrison, P. J. (1982). Comparison of Solvents for Extracting Chlorophylls from Marine Macrophytes. Botanica Marina, 25(9). doi:10.1515/botm.1982.25.9.445
Methods

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Parameters

ParameterDescriptionUnits
Label

Trial number-pot number.frozen tissue replicate

unitless
pCO2_avg

Average pCO2 partial pressure in seawater tanks

microatmospheres (µatm)
pCO2_sd

Variability of pCO2 partial pressure - standard deviation

microatmospheres (µatm)
Fresh_Wt

Fresh weight of tissue sample prior to deep freeze

grams
DMSO_A665

Absorbance of light by ground Ulva tissue at 665nm in 1.25 ml of an 80% DMSO solution in water

unitless
Methanol_A668

Absorbance of light by ground Ulva tissue at 668nm in 6 ml methanol

unitless
Methanol_A635

Absorbance of light by ground Ulva tissue at 635nm in 6 ml methanol

unitless
DMSO_ext

Chlorophyll a extracted in DMSO

milligram/liter (mg/L)
Meth_ext

Chlorophyll a in pooled extracts of 12 ml of methanol

milligram/liter (mg/L)
Total_chl_mg

Total chlorophyll a extracted from ground tissue

milligrams (mg)
chl_a

Chlorophyll a concentration per unit biomass

milligrams/gram (mg/g)


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Instruments

Dataset-specific Instrument Name
Shimadzu UV-2450 UV-visible spectrophotometer
Generic Instrument Name
UV Spectrophotometer-Shimadzu
Dataset-specific Description
Used to measure chlorophyll a concentration.
Generic Instrument Description
The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.


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Project Information

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Coverage: Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)


Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.



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Program Information

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)


Coverage: global


NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).

In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.

Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.

PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)

NSF media releases for the Ocean Acidification Program:

Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification

Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?

Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)

Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)

Press Release 13-102 World Oceans Month Brings Mixed News for Oysters

Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)

Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants

Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)

Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)

Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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