Dataset: HPLC pigments WB
Deployment: WB0409

HPLC pigments, EDDIES WB cruises

Principal Investigator:

Dennis J. McGillicuddy (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Eddies Dynamics, Mixing, Export, and Species composition (EDDIES)

Version:

13 March 2007

Deployment Synonyms:

EDT1 2004 Transect 1

Version Date:

2007-03-13

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/452234/data

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Description

Pigments from HPLC analysis of bottle samples collected during EDDIES Weatherbird cruises

 dates:           2004 - 2005
 location:        Sargasso Sea 
 project/cruise:  EDDIES/WB0409 2004 Transect 1 (EDT1)
                  EDDIES/WB0413 2004 Transect 2 (EDT2)
                  EDDIES/WB0506 2005 Transect 1 (EDT3)
                  EDDIES/WB0508 2005 Transect 2 (EDT4)
 platform:        R/V Weatherbird II


 Change history: YYMMDD
    070308: downloaded original data from EDDIES data Web site 
            (200609 DM FINAL_corrected.xls);
            prepared for OCB database by Nancy Copley, OCB DMO
    070313: added to OCB database by Cyndy Chandler, OCB DMO
            event number is from cruise event log
    070405: per Jason Perl, units for all pigment concentrations  
            changed from microgram/kilogram to microgram/liter 
 
 PI note: 080317: Recalibrated data are expected from NASA during the second half of 2008.

Methods & Sampling

Dataset acquisition description

Methodology, protocols and PI-notes: NASA/TM-2003- HPLC Protocols (1 MB PDF)
J.L. Mueller, G.S. Fargion, C.R. McClain, C.C. Trees and J. Perl. 2003. "Ocean Optics Protocols For Satellite Ocean Color Sensor Validation, Revision 5, Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Protocols". NASA Tech. Memo., NASA Goddard Space Flight Center, Greenbelt, Maryland. 43 pp. (PDF downlaoded from http://oceancolor.gsfc.nasa.gov/DOCS/)

Technician: Jason Perl (jperl@chors.sdsu.edu)
Center for Hydro-Optics & Remote Sensing
San Diego State University Research Foundation

Technician notes:
note 1: Did not have a signal for Chl_a @ 450nm (sample too dilute to get a chromatogram peak)
note 2: Did not have a signal for Chl_a @ 450nm or 436nm (sample too dilute to get a chromatogram peak)
note 3: no ancillary data; default volume filter set to 4000mL

chl_a_fluor and pheo_fluor are values from a Turner Fluorometer. Each HPLC extract is run on the Fluorometer as a second measure of Chla. Chla, MVa, DVa using the C18 HPLC method, yield a single peak for Chla, measured at 436nm and 450nm. A regression equation, between the 2 wavelengths (based on calibration curves of 100% MVa and 100% DVa), is used to give values for each pigment within a single peak. Chla @436nm is reported for comparison with legacy data (that are often only listed simply Chla).

Data Processing Description

Dataset Processing Description

DMO notes:
Original Excel file downloaded from EDDIES Web site: copy of original Excel file

The comments in the data object were taken from the 061127 version of file readme_jason_notes.txt.
SID_Filter and SID_PI were identical but some samples were also included another sample designation that was a consecutive SID_PI. In these cases, the consecutive SID replaced the SID_PI.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0241310	NSF Division of Ocean Sciences

Instruments

Niskin bottle

Supplied Name: Niskin Bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise_ID	cruise ID designation code	alphanumeric	cruise_id
event	unique sampling event number in the format YYYYMMDDhhmm	unitless	event
date	date (GMT); start of sampling in the format YYYYMMDD	unitless	date
time	time of measurement (GMT) in the format hhmm	unitless	time
lon	longitude at start of measurement; negative denotes West	decimal degrees	lon
lat	latitude at start of measurement; negative denotes South	decimal degrees	lat
sta	station number	dimensionless	sta
depth_n	depth; nominal	meters	depth_n
run_ID_HPLC	sample run ID given by HPLC lab	alphanumeric	sample
run_PI	sample run ID given by PI	alphanumeric	sample
run_filt_ID	sample run filter ID	alphanumeric	sample
vol_filt_ml	volume water filtered for analysis	milliliters	vol_filt
chl_a_fluor	chlorophyll-a by Fluorometric methods	microgram/liter	chl_a_fluor
pheo_fluor	pheopigments measured by Fluorometric methods	microgram/liter	phaeo
chlide_a	HPLC Chlorophyllide a	microgram/liter	chlide_a
phide	HPLC Pheophorbide a	micrograms/liter	p_phorbide
pheo	HPLC Pheophytin a	microgram/liter	p_phytin
chl_a_allomer	HPLC chlorophyll a allomer	microgram/liter	chl_a_allo
chl_a	HPLC chlorophyll a (measured at 436nm)	microgram/liter	chl_a
chl_a_epimer	HPLC chlorophyll a epimer	microgram/liter	chl_a_epimer
chl_c3	HPLC Chlorophyll_c3	microgram/liter	chl_c3
chl_c2	HPLC Chlorophyll_c2	microgram/liter	chl_c2
peridinin	HPLC Peridinin	microgram/liter	peridinin
fucox_but	HPLC 19' - Butanoyloxyfucoxanthin	microgram/liter	fucox_but
fucox	HPLC fucoxanthin	microgram/liter	fucox
fucox_hex	HPLC 19' - Hexanoyloxyfucoxanthin	microgram/liter	fucox_hex
neox	HPLC neoxanthin	microgram/liter	neox
prasinox	HPLC Prasinoxanthin	microgram/liter	prasinox
violax	HPLC violaxanthin	microgram/liter	violax
diadinox	HPLC Diadinoxanthin	microgram/liter	diadinox
allox	HPLC Alloxanthin	microgram/liter	allox
diatox	HPLC Diatoxanthin	microgram/liter	diatox
lutein	HPLC Lutein	microgram/liter	lutein
zeax	HPLC Zeaxanthin	microgram/liter	zeax
chl_b	HPLC Chlorophyll b	microgram/liter	chl_b
carotene	HPLC Total Carotenes (alpha + beta)	microgram/liter	carotene
chl_a_mv	Monovinyl chlorophyll a; based on the C18 method regression between 436nm and 450nm	microgram/liter	chl_a1
chl_a_dv	Divinyl chlorophyll a; based on the C18 method regression between 436nm and 450nm	microgram/liter	chl_a2
chl_a_tot	Total Chlorophyll a =chlide_a +allomer +epimer +MVa +Dva	microgram/liter	chl_a_tot
comments	comments and notes	text	comment

Database

Contribute Data

Dataset: HPLC pigments WB
Deployment: WB0409

Pigments from HPLC analysis of bottle samples collected during EDDIES Weatherbird cruises

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: HPLC pigments WBDeployment: WB0409

Pigments from HPLC analysis of bottle samples collected during EDDIES Weatherbird cruises

Dataset acquisition description

Dataset Processing Description

Dataset: HPLC pigments WB
Deployment: WB0409