Dataset: Picoplankton
Deployment: KNOX22RR

Picoplankton Abundance Data

Principal Investigator:

Dr Lisa R. Moore (University of Southern Maine, USM)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Coccolithophores of the Patagonian Shelf 2008 (COPAS08)

Current State:

Final no updates expected

Version:

14 May 2010

Deployment Synonyms:

COPAS08

Version Date:

2010-05-14

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/453077/data

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Description

Picoplankton abundance data from the COPAS'08 cruise

Population abundances of Prochlorococcus, Synechococcus, picoeukaryotic phytoplankton and non-chlorophyll containing prokaryotes, "heterotrophic bacteria", analyzed from collected flow cytometry samples.

Methods & Sampling

Dataset acquisition description

The primary goal of the Moore lab on the COPAS cruise was to study the horizontal
and vertical abundance distributions of the picophytoplankton populations along and
across the Patagonia Shelf region with a particular emphasis on the picocyanobacteria.
We collected water samples from roughly 25 stations at five depths: three within the
surface mixed layer, at the chlorophyll max, and immediately below the chlorophyll max.
At each of these five depths 1 mL of water was preserved with 0.125% glutaraldehyde
and frozen in liquid nitrogen for subsequent flow cytometric analysis of the
picophytoplankton populations back at USM. From the carboy CO2 incubation experiments
we preserved water at T0, T24 and T72 from all CO2 pressures for flow cytometric
analysis of the picophytoplankton populations response to increased CO

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes
Generated from original text file "copas08_picoplankton_14may2010.xlsx" contributed by Lisa Moore

BCO-DMO Edits
- Parameter names modified to conform to BCO-DMO convention
- date/time added from CTD header data/stations
- lat/lons standardized to lat/lons in CTD data for consistency
- blank cells changed to "nd"

More information about this dataset deployment

Funding

Award Number	Funding Source
JGS 1166	Defence Science and Technology Laboratory
NNX08AJ88A	National Aeronautics & Space Administration
OCE-0728582	NSF Division of Ocean Sciences

Instruments

CTD Sea-Bird 911

Supplied Description:

Data were acquired using SeaBird SeaSave v7 for SBE 911 software

Instrument Type

Generic Name: CTD Sea-Bird 911

Acronym: CTD SBE 911

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/

Generic Description:

The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics.

Flow Cytometer

Supplied Description:

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Niskin bottle

Supplied Name: Niskin Bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
station	COPAS'08 Station Id	text	station
date	date (GMT)	YYYYMMDD	date
time	time (GMT)	HHMMSS	time
lon	Station longitude (West is negative)	decimal degrees	lon
lat	Station latitude (South is negative)	decimal degrees	lat
Depth	Sample depth	meters	depth
Syn	Synechococcus	cells/ml	coccus_s
Pro	Prochlorococcus	cells/ml	coccus_p
Pico_Euk	picoeukaryotic phytoplankton	cells/ml	no_bcodmo_term
Total_Cyano	Total Cyanophores	cells/ml	no_bcodmo_term
Total_picophytoplankton	Total picophytoplankton	cells/ml	no_bcodmo_term
HB	non-chlorophyll containing prokaryotes (heterotrophic bacteria)	cells/ml	hb

Database

Contribute Data

Dataset: Picoplankton
Deployment: KNOX22RR

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: PicoplanktonDeployment: KNOX22RR

Dataset acquisition description

Dataset Processing Description

Dataset: Picoplankton
Deployment: KNOX22RR