Dataset: Pigments
Deployment: KN192-05

HPLC analyses of algal pigment concentrations.

Principal Investigator:

Giacomo DiTullio (Grice Marine Laboratory - College of Charleston, GML-CoC)

Contact:

Giacomo DiTullio (Grice Marine Laboratory - College of Charleston, GML-CoC)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Cobalt, Iron and Micro-organisms from the Upwelling zone to the Gyre (GAc01) (CoFeMUG)

Current State:

Preliminary and in progress

Version:

18 May 2011

Deployment Synonyms:

GAc01, CoFeMUG, KN192-5

Version Date:

2011-05-18

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/454583/data

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Description

HPLC analyses of algal pigment concentrations from the CoFeMUG cruise.

Methods & Sampling

Dataset acquisition description

Algal HPLC pigment samples (2L-4L) were collected from CTD profiles, filtered under low vacuum through GF/F filters, and frozen in LN for onshore analyses. Samples were extracted overnight in cold (-20 degrees C) 100% acetone and analyzed using an Agilent 1100 Model HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program used was a slight modification of the Zapata et al. gradient elution method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002). Detection limits for the pigments were on the order of 1 nanogram with a coefficient of variation of <3% for replicate HPLC pigment standard injections. Pigments were isolated from unialgal cultures grown in our lab.

DITULLIO, G. R., AND M. E. GEESEY. 2002. Photosynthetic pigments in marine algae and bacteria, p. 2453 - 2470.In G. Bitton [ed.], The Encyclopedia of Environmental Microbiology. Wiley.

ZAPATA, M., F. RODRIGUEZ, AND J. L. GARRIDO. 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Mar. Ecol. Prog. Ser. 195: 29 - 45.

Data Processing Description

Dataset Processing Description

Standard curves relating pigment concentration to peak area were run for each pigment.

Related files and references:
DITULLIO, G. R., AND M. E. GEESEY. 2002. Photosynthetic pigments in marine algae and bacteria, p. 2453 - 2470.In G. Bitton [ed.], The Encyclopedia of Environmental Microbiology. Wiley.

BCO-DMO Processing Notes
Generated from original .xls file 'CoFeMug Station Pigs.xls' contributed by Tyler Cyronak

BCO-DMO Edits
- Parameter names modified to conform to BCO-DMO convention
- Date reformatted to YYYYMMDD
- Time (GMT and Local) reformatted to HHMM
- Blank lines removed
- lat deg/min and lon deg/min columns removed (lat/lon in decimal degrees preserved)
- 'nd' (no data) value inserted in blank cells
- '-999' bad data flag replaced with 'nd'

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0728683	NSF Division of Ocean Sciences

Instruments

CTD Sea-Bird SBE 911plus

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird SBE 911plus

Acronym: CTD SBE 911plus

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/

Generic Description:

The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics

Niskin bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
station	Station number	integer	station
cast	Cast number	integer	cast
date	date (GMT)	YYYYMMDD	date
time_start_gmt	Start time (GMT)	HHMM	time_start
time_start_local	Start time (Local)	HHMM	time_start_local
lat	Station latitude (South is negative)	decimal degrees	lat
lon	Station longitude (West is negative)	decimal degrees	lon
depth	Sample depth	meters	depth
Niskin	Niskin bottle number	integer	bottle
Amb_Bot	Ambient bottle number	integer	no_bcodmo_term
Fluoro_Chl_a_Conc	Fluoro_Chl_a_Conc	ug/L	no_bcodmo_term
MgDVP	MgDVP	ng L-1	no_bcodmo_term
Chl_c2	Chl_c2	ng L-1	chl_c2
Chl_c1	Chl_c1	ng L-1	chl_c1
Peridinin	Peridinin	ng L-1	peridinin
But_19	19_But	ng L-1	no_bcodmo_term
Fuco	Fuco	ng L-1	no_bcodmo_term
Neo	Neo	ng L-1	no_bcodmo_term
Prasino	Prasino	ng L-1	no_bcodmo_term
Viola	Viola	ng L-1	no_bcodmo_term
Hex_19	19_Hex	ng L-1	no_bcodmo_term
Diadino	Diadino	ng L-1	no_bcodmo_term
Cis_Fuco	Cis_Fuco	ng L-1	no_bcodmo_term
Allo	Allo	ng L-1	no_bcodmo_term
Diato	Diato	ng L-1	no_bcodmo_term
Zeaxanthin	Zeaxanthin	ng L-1	no_bcodmo_term
Lutein	Lutein	ng L-1	lutein
Crocoxanthin	Crocoxanthin	ng L-1	no_bcodmo_term
DV_Chl_a	DV_Chl_a	ng L-1	no_bcodmo_term
Chl_a	Chl_a	ng L-1	chl_a
Chlc_Like	Chlc_Like	ng L-1	no_bcodmo_term
a_Car	a_Car	ng L-1	no_bcodmo_term
b_Car	b_Car	ng L-1	no_bcodmo_term
Chl_c3	Chl_c3	ng L-1	chl_c3
Chl_b	Chl_b	ng L-1	chl_b
Chl_cMVP	Chl_cMVP	ng L-1	no_bcodmo_term
Phaeophorobide	Phaeophorobide	ng L-1	no_bcodmo_term
Phaeophytin	Phaeophytin	ng L-1	no_bcodmo_term

Database

Contribute Data

Dataset: Pigments
Deployment: KN192-05

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: PigmentsDeployment: KN192-05

Dataset acquisition description

Dataset Processing Description

Dataset: Pigments
Deployment: KN192-05