Dataset: chlorophyll_a
Deployment: DMSP_Dauphin_Island

Chlorophyll-a concentration in microcosm experiments.

Principal Investigator:

Mary Ann Moran (University of Georgia, UGA)

Co-Principal Investigator:

Ronald P. Kiene (Dauphin Island Sea Lab, DISL)

William Whitman (University of Georgia, UGA)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the Ocean (En-Gen DMSP Cycling )

Current State:

Final no updates expected

Version:

19 Nov 2012

Version Date:

2012-11-19

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/455723/data

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Description

Chlorophyll-a measurements from control and experimental microcosms from the Dauphin Island Cubitainer Experiment (DICE).

Experimental design, methods, and results are further described in: Howard et al. (2010) and Rinta-Kanto et al. (2011).

Methods & Sampling

Dataset acquisition description

See Howard et al. 2010 and Rinta-Kanto et al. 2011 for detailed methods, summarized below:

"In October 2006, seawater was collected from surface waters (<1 m deep) in the Gulf of Mexico off the coast of Dauphin Island, AL (lat: 30 03.041N; lon: 87 59.708W). Water was filtered through a 200-um mesh into six 20-liter polyethylene Cubitainers with minimal headspace.

Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three microcosms were left untreated to serve as the control. The Cubitainers were maintained at 27 degrees C on a 12-hour light/dark cycle for the duration of the experiment.

Chemical and activity measurements were collected from the microcosms at the beginning of the experiment (Day 0) and every day for the duration of the experiment at the same time. Chlorophyll-a samples were collected by filtration on Whatman GF/F fiters. The filters were extracted in 90% acetone for 24 hours at -20 degrees C. The extracts were quantified by fluorometry on a Turner Designs TD-700."

Data Processing Description

Dataset Processing Description

BCO-DMO made the following changes:
- Modified parameter names to conform with BCO-DMO naming conventions.
- Replaced blanks with 'nd' to indicate 'no data'.
- Separated date in day, month, and year columns.
- Added the site coordinates provided in the publications above.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0724017	NSF Division of Ocean Sciences

Instruments

bucket

Supplied Description:

Water was collected in the field using a clean bucket.

Instrument Type

Generic Name: bucket

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0536/

Generic Description:

A bucket used to collect surface sea water samples.

Turner Designs 700 Laboratory Fluorometer

Supplied Description:

A Turner Designs TD-700 was used.

Instrument Type

Generic Name: Turner Designs 700 Laboratory Fluorometer

Acronym: TD-700

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/

Generic Description:

The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lat	Latitude of the collection site. North = Positive.	decimal degrees	lat
lon	Longitude of the collection site. West = Negative.	decimal degrees	lon
site_desc	Description of the sample collection site.	text	site_descrip
exp_day	Day of the experiment. First experimental day = T0.	unitless	no_bcodmo_term
month	2-digit month of year.	mm (01 to 12)	month
day	2-digit day of month.	dd (01 to 31)	day
year	4-digit year in YYYY format.	unitless	year
microcosm	Identifier of the microcosm. C0 and E0 are the time zero (initial) samples that represent the starting chl-a concentrations in the seawater.	unitless	sample
microcosm_type	Type of microcosm: experimental or control.	text	sample_descrip
chl_a_mean	Mean chlorophyll a per microcosm per day.	micrograms/Liter	chl_a_fluor
chl_a_sd	Standard deviation of chl_a_mean.	micrograms/Liter	no_bcodmo_term
replicate	Identifier of the technical replicate (pulled from the same microcosm).	unitless	replicate
vol_filt	Volume of water filtered measured in milliliters.	mL	fluor_vol_filt
vol_ext	Volume extracted in milliliters.	mL	no_bcodmo_term
fluor	Fluorometer reading. (The fluorometer excites the sample with blue light and red fluorescence is measured. The maximum emission is 668 nm.)	fluorometer reading	no_bcodmo_term
chl_a	Chlorophyll-a.	micrograms/Liter	chl_a_fluor
exp_id	Name of the experiment. DICE = Dauphin Island Cubitainer Experiment.	text	exp_id

Database

Contribute Data

Dataset: chlorophyll_a
Deployment: DMSP_Dauphin_Island

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: chlorophyll_aDeployment: DMSP_Dauphin_Island

Dataset acquisition description

Dataset Processing Description

Dataset: chlorophyll_a
Deployment: DMSP_Dauphin_Island