Dataset: Sargasso Sea surface bacteria accession numbers
Deployment: AE1006

Paired metagenomic and metatranscriptomic data from 2 cruises in the spring of 2010 and the summer of 2010 in the Sargasso Sea

Principal Investigator:

Dr Barbara Campbell (Clemson University, Clemson)

Co-Principal Investigator:

Dr John Heidelberg (University of Southern California, USC)

David L. Kirchman (University of Delaware)

Student:

Rohan Sachdeva (University of Southern California, USC)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Are abundant bacteria more active than rare bacteria in the Sargasso Sea? (Active bacteria in surface waters)

Current State:

Final no updates expected

Deployment Synonyms:

256, B256, GF256

Version Date:

2014-04-23

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/512786/data

Expand/Collapse All

Description

Methods & Sampling

Dataset acquisition description

A combination of pyrosequencing and QPCR approaches were used to examine rRNA:rDNA ratios, BrdU incorporating cells, and transcript types and amounts in the metatranscriptome of Sargasso Sea surface water. The investigators also used Micro-FISH to examine incorporation of thymidine, leucine, and PO4. Samples were collected in 2010 during the spring phytoplankton bloom when heterotrophic bacterial production is lowest and during the peak of bacterial production in summer.

Data Processing Description

Dataset Processing Description

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0824981	NSF Division of Ocean Sciences
OCE-0825468	NSF Division of Ocean Sciences

Instruments

Niskin bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cruise identification; AE is Atlantic Explorer	unitless	cruise_id
date	date of collection	mm/dd/yyyy	date
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
depth	depth of sample	meters	depth
accession_number	NCBI accession number	unitless	accession_number

Database

Contribute Data

Dataset: Sargasso Sea surface bacteria accession numbers
Deployment: AE1006

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Sargasso Sea surface bacteria accession numbersDeployment: AE1006

Dataset acquisition description

Dataset Processing Description

Dataset: Sargasso Sea surface bacteria accession numbers
Deployment: AE1006