Dataset: recruit_growth_area
Deployment: lab_Edmunds_NMMBA

Coral recruit growth as a function of corallite area change during CO2 experiments

Lead Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Student:

Aaron M. Dufault (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Current State:

Preliminary and in progress

Version:

2014-03-07

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/514201/data

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Description

Manipulative studies have demonstrated that ocean acidification (OA) is a threat to coral reefs, yet no experiments have employed diurnal variations in pCO2 that are ecologically relevant to many shallow reefs. Two experiments were conducted to test the response of coral recruits (less than 6 days old) to diurnally oscillating pCO2; one exposing recruits for 3 days to ambient (440 uatm), high (663 uatm) and diurnally oscillating pCO2 on a natural phase (420–596 uatm), and another exposing recruits for 6 days to ambient (456 uatm), high (837 uatm) and diurnally oscillating pCO2 on either a natural or a reverse phase (448–845 uatm).

These data are published in Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Related Datasets:

recruit_seawater_chemistry
recruit_growth_weight
recruit_suvival

Methods & Sampling

Dataset acquisition description

Larvae were obtained from brooding colonies coral S. caliendrum collected from 5 to 7 m deep on Hobihu Reef, Nanwan Bay, in March and June of 2010, placed into individual flow-through seawater tanks. Overflow water from each tank passed through mesh-lined (110 mm) cups that captured larvae. Following collection, larvae were settled onto clean pre-weighed glass microscope coverslips. Coverslips with coral recruits (n=18: experiment I; n=36: experiment II) were assigned randomly to the pCO2 treatments.

In experiment I, treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase; this design was augmented in experiment II by including a diurnally oscillating pCO2 on a reverse phase.

To measure the area of the recruits, bleached and dried corallites were photographed (Nikon Coolpix 4500, 4.0 megapixel resolution) through a compound microscope (Zeiss Axiolab E, 40 magnification).

For detailed description of methods, see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Data Processing Description

Dataset Processing Description

Images were analysed using the IMAGE J software (Wayne Rasband, v. 1.42q), with approximately 65 corallites in each treatment measured for experiment I, and approximately 33 in each treatment for experiment II. To obtain an unambiguous indication of area, only corallites that were not touching neighboring corallites were selected for analysis, and each corallite was treated as a statistical replicate.

For detailed description of processing see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0844785	NSF Division of Ocean Sciences

Instruments

Camera

Supplied Name: camera

Supplied Description:

A Nikon Coolpix 4500, 4.0 megapixel resolution camera was used to photograph recruit area.

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
laboratory	Laboratory where experiments were conducted.	unitless	laboratory
experiment	Experiment identifier: Experiment I treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase. Experiment II treatments consisted of a diurnally oscillating pCO2 on a reverse phase.	dimensionless	exp_id
lat	Latitude component of geographic position where experiments were conducted.	decimal degrees	lat
lon	Longitude component of geographic position where experiments were conducted.	decimal degrees	lon
duration	Experiment duration: 3 (Exp I) or 6 days (Exp. II).	days	time_inc
coverslip	Unique coverslip the coral was settled upon.	dim	sample
treatment_CO2	Individual CO2 treatment (A=ambient, H=High, D=Diurnal, DX=Reverse-phase Diurnal). Originally reported as "CO2".	dimensionless	treatment
tank	Unique tank the coverslips with settled corals were placed in (a decimal number represents 2 tanks used for the diurnal treatments - corals were placed in the first tank during the day (1st number) and into the second tank at night (2nd number), eg Tank 1.8= tank 1 during day, tank 8 at night).	dimensionless	tank
month	Month of coral colony collection.	dimensionless	month
year	Year of coral colony collection.		year
area	Area of corallite.	millimeters^2	no_bcodmo_term
area_day	Area of corallite normalized to the duration of the experiment.	millimeters^2 per day	no_bcodmo_term
transformation	Square root transformation to address negative skewing in Experiment II.	unknown	no_bcodmo_term

Database

Contribute Data

Dataset: recruit_growth_area
Deployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: recruit_growth_areaDeployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Dataset: recruit_growth_area
Deployment: lab_Edmunds_NMMBA