Dataset: brooded coral larvae 2 - carbonate chemistry
Deployment: lab_Edmunds_NMMBA

Seawater carbonate chemistry for brooded coral larval experiments, March 2011 & 2012 (Cumbo, 2013)

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Scientist:

Dr Vivian R Cumbo (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Current State:

Final no updates expected

Version:

2014-08-30

Version Date:

2014-08-30

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/528812/data

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Description

To evaluate the effects of temperature and pCO2 on coral larvae, brooded larvae of Pocillopora damicornis from Nanwan Bay, Taiwan (21°56.179' N, 120°44.85' E), were exposed to ambient (419-470 µatm) and high (604-742 µatm) pCO2 at ~25 and ~29 °C in two experiments conducted in March 2010 and March 2012. Larvae were sampled from four consecutive lunar days (LD) synchronized with spawning following the new moon, incubated in treatments for 24 h, and measured for respiration, maximum photochemical efficiency of PSII (F v/F m), and mortality.

The most striking outcome was a strong effect of time (i.e., LD) on larvae performance: respiration was affected by an LD × temperature interaction in 2010 and 2012, as well as an LD × pCO2 × temperature interaction in 2012; F v/F m was affected by LD in 2010 (but not 2012); and mortality was affected by an LD × pCO2 interaction in 2010, and an LD × temperature interaction in 2012. There were no main effects of pCO2 in 2010, but in 2012, high pCO2 depressed metabolic rate and reduced mortality. Therefore, differences in larval performance depended on day of release and resulted in varying susceptibility to future predicted environmental conditions. These results underscore the importance of considering larval brood variation across days when designing experiments. Subtle differences in experimental outcomes between years suggest that transgenerational plasticity in combination with unique histories of exposure to physical conditions can modulate the response of brooded coral larvae to climate change and ocean acidification.

These data include the seawater carbonate chemistry monitored from the experimental tanks, March 2011 and 2012.

Related datasets:

brooded coral larvae 2 - larval release March 2003-2008
brooded coral larvae 2 - respiration_photosyth_mortality

These data are published in Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. (2013) Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release. Marine Biology. See Table 1.

Download complete data for this publication (Excel file)
Data also available from PANGAEA: DOI 10.1007/s00227-013-2280-y

Methods & Sampling

Dataset acquisition description

To determine the dissolved inorganic carbon (DIC) chemistry of seawater in the treatments, total alkalinity (TA), pH on the total scale, temperature, and salinity were measured using standard procedures, and used to calculate pCO2 (uatm), HCO3 - (umolkg-1), CO3 2- (umolkg-1), and the aragonite saturation state (XArag) of seawater using CO2SYS (Pierrot et al. 2006). TA (umol kg-1) was measured through potentiometric titration (SOP 3b, Dickson et al. 2007) using an automatic titrator (DL50, Mettler Toledo) filled with certified acid titrant (0.1 M HCl, 0.6 NaCl, Dickson Laboratory, Scripps Institution of Oceanography). The pH probe (DG101-SC, Mettler Toledo) attached to the titrator was 3-point calibrated with pH 4.00, 7.00, and 10.00 buffers (Fisher, NBS). Certified reference material with a known TA (Batch 98, http://andrew.ucsd. edu/index.html) was titrated daily to determine the accuracy and precision of the analyses.

Seawater samples (50 mL) from the treatment tanks were brought to 25C, weighed and titrated in a waterjacketed beaker within 2-3 h of collection. The pH values and the titrant volumes (cm3) obtained from the titrations were sub-sampled for the range between pH 3.0 and 3.5 and inserted into a Microsoft Excel spreadsheet (Fangue et al. 2010), which calculated Gran’s function as a product of the mass of titrant added (Dickson et al. 2007). Treatment tank pH was determined spectrophotometrically using m-cresol purple dye (Sigma-Aldrich) following SOP 6b of Dickson et al. (2007) with modification (Fangue et al. 2010). Preliminary sampling of the seawater in the tanks throughout the day confirmed that the pCO2 treatments were stable over a 24-h period.

References:

Dickson AG, Sabine CL, Christian JR (eds) (2007) Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, p 191

Fangue NA, O’Donnell MJ, Sewell MA, Matson PG, MacPherson AC, Hoffman GE (2010) A laboratory-based, experimental system for the study of ocean acidification effects on marine invertebrate larvae. Limnol Oceanogr Methods 8:441-452

Data Processing Description

Dataset Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- added lab, lat, lon, expt columns
- renamed parameters to BCO-DMO standard
- combined data from Table 1, 2012 and 2012 - sorted by date, temp, treatment

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0844785	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Description:

Model DL50, Mettler-Toledo, Columbus, OH, USA

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
expt	experiment id	unitless	exp_id
lab	laboratory	unitless	laboratory
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
month	experiment month	unitless	month
tank	tank id number	tank	tank
date	local date of measurement	yyyy-mm-dd	date
pH	pH: The measure of the acidity or basicity of an aqueous solution	pH scale	pH
temp	temperature	degees Celsius	temp
sal	salinity	PSU scale	sal
TA	total alkalinity	umol/kg	TALK
pCO2	partial pressure of carbon dioxide by computation from pH and alkalinity	uatm	pCO2
bicarbonate	concentration of bicarbonate ion ([HCO3]-) in seawater	umol/kg	bicarbonate
carbonate	concentration of carbonate ion ([CO3]2-)	umol/kg	carbonate
omega_Ara	the saturation state of seawater with respect to aragonite	unitless	OM_ar

Database

Contribute Data

Dataset: brooded coral larvae 2 - carbonate chemistry
Deployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: brooded coral larvae 2 - carbonate chemistryDeployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Dataset: brooded coral larvae 2 - carbonate chemistry
Deployment: lab_Edmunds_NMMBA