Dataset: brooded coral larvae 3 - symbiont density
Deployment: lab_Edmunds_NMMBA

Symbiont Symbiodinium density in brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013)

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Co-Principal Investigator:

Dr Vivian R Cumbo (California State University Northridge, CSUN)

Tung-Yung Fan (National Museum of Marine Biology and Aquarium, NMMBA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Current State:

Final no updates expected

Version:

2014-10-07

Version Date:

2014-10-07

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/535365/data

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Description

Methods & Sampling

Dataset acquisition description

Symbiodinium densities were determined by preserving 2 groups of 6 larvae fromeach tank (one from each tub) in 100 uL of 10% formalin in FSW, and macerating them with a Teflon pestle. The Symbiodinium in the slurry were counted using a hemocytometer (4 replicate counts) and the algal density expressed as cells mg protein-1. Preliminary analyses demonstrated that the mean and SE of the Symbiodinium counts stabilized at 4 replicate counts, and therefore additional replicate counts were not considered necessary. Quality control was conducted by screening the data for cases where the SE of the counts was >20% of the mean, and in rare cases where this occurred, counts were repeated.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582

Data Processing Description

Dataset Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- reformated date from d-Mon-yy to yyyy-mm-dd
- changed Ambient to ambient; High to high; P. dam to Pocillopora_damicornis
- reduced number of significant digits
- replaced blanks with underscores

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0844785	NSF Division of Ocean Sciences

Instruments

Aquarium chiller

Supplied Description:

Aquatech Ac11 or Shyeh Duwai Enterprise

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Gas Analyzer

Supplied Description:

Infra Red gas analyzer (S151, Qubit Systems)

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Hemocytometer

Supplied Description:

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Immersion heater

Supplied Description:

300 Wheaters, Taikong Corporation

Instrument Type

Generic Name: Immersion heater

Generic Description:

Submersible heating element for water tanks and aquaria.

LI-COR LI-192 PAR Sensor

Supplied Name: LI-COR LI-192 light sensor

Supplied Description:

cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)

Instrument Type

Generic Name: LI-COR LI-192 PAR Sensor

Acronym: LI-COR LI-192 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/

Generic Description:

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

Optode

Supplied Name: optrode

Supplied Description:

A Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature.

Instrument Type

Generic Name: Optode

Generic Description:

An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

Water Temperature Sensor

Supplied Name: Water Temp Sensor

Supplied Description:

certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory	unitless	laboratory
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
date	local date	yyyy-mm-dd	date
sample	sample identification	unitless	sample
tank	tank id number	tank	tank
temp	target temperature	degrees Celsius	temp
treatment_pCO2	pCO2 treatment: ambient (47.5 - 49.3 Pa) or high (85.2 - 87.2 Pa)	unitless	treatment
days	days since start of experiment	unitless	days
num_larvae	number of larvae examined	integer	count
vol_chamber	the volume in which the cells were resuspended prior to counting	ml	sample_volume
vol_grid	volume of grid	ml	sample_volume
rep_1	number of symbionts in replicate 1 of hemocytometer slide	integer	count
rep_2	number of symbionts in replicate 2 of hemocytometer slide	integer	count
rep_3	number of symbionts in replicate 3 of hemocytometer slide	integer	count
rep_4	number of symbionts in replicate 4 of hemocytometer slide	integer	count
count_mean	average number of symbionts in samples	symbionts	count
count_stdev	standard deviation: number of symbionts in samples	symbionts	std_dev
count_se	standard error: number of symbionts in samples	symbionts	std_err
CV	coefficient of variation: a ratio of the standard deviation (number of symbionts per sample) to the mean (number of symbionts per sample).	percent: unitless	no_bcodmo_term
cells_total	total number of symbionts from the 4 replicates	symbionts	count
cells_larva	average number of symbionts per larva	cells/larva	count
protein_total	total protein from all 6 larvae in incubation chamber	milligrams (mg)	no_bcodmo_term
cells_mg_protein	number of symbionts per milligram protein	cells/mg protein	no_bcodmo_term

Database

Contribute Data

Dataset: brooded coral larvae 3 - symbiont density
Deployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: brooded coral larvae 3 - symbiont densityDeployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Dataset: brooded coral larvae 3 - symbiont density
Deployment: lab_Edmunds_NMMBA