Dataset: Montipora chemical fingerprints
Deployment: Aeby_2014

Chemical fingerprints of Montipora capitata aqueous extracts

Principal Investigator:

Deborah J. Gochfeld (University of Mississippi, UM-NCNPR)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Host-environment-pathogen interactions in a model coral disease system (coral-pathogen interaction)

Current State:

Preliminary and in progress

Version:

2014-08-28

Version Date:

2014-08-28

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/544909/data

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Description

Data access is restricted. Please contact the PI for further information.

Related datasets:

Montipora antibacterial-aqueous
Montipora antibacterial-mucus
Montipora antibacterial-organic
MWS accession numbers
MWS lesion progression

Related references:

Deborah J. Gochfeld & Haidy N. Kamel & Julie B. Olson & Robert W. Thacker. 2012. Trade-offs in defensive metabolite production but not ecological function in healthy and diseased sponges. J Chem Ecol 38:451-462. DOI 10.1007/s10886-012-0099-5.

Methods & Sampling

Dataset acquisition description

Preparation of extracts: Coral samples of orange and red colonies of Montipora capitata were collected in Kaneohe Bay, Oahu. Frozen samples were extracted in Millipore® water, which was replaced daily for 3 d. Extracts were filtered, lyophilized, and weighed. Extracts were resuspended in Millipore® water to a concentration of 10 mg/ml.

Chemical fingerprinting: To generate chemical profiles, extracts were injected onto a Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector. Spectra at 254 nm were generated. Each peak observed at the same retention time in each extract represents a particular compound, relative concentrations of which can be compared between extracts.

Data Processing Description

Dataset Processing Description

Peaks from the 254 nm spectra were integrated to generate areas under the curve (in absorbance units). These areas can then be compared among extracts.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard or to match other datasets in project
- replaced blanks with underscores
- replaced color_morph code with color name: 1=orange; 2=red

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961384	NSF Division of Ocean Sciences

Instruments

High-Performance Liquid Chromatograph

Supplied Name: HPLC

Supplied Description:

Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
color_morph	color morph of coral	unitless	no_bcodmo_term
coral	coral fragment identification	unitless	sample
peak_1	Relative concentration of coral extract compound from peak 1 from the 254 nm spectra	unitless	no_bcodmo_term
peak_2	Relative concentration of coral extract compound from peak 2 from the 254 nm spectra	unitless	no_bcodmo_term
peak_3	Relative concentration of coral extract compound from peak 3 from the 254 nm spectra	unitless	no_bcodmo_term
peak_4	Relative concentration of coral extract compound from peak 4 from the 254 nm spectra	unitless	no_bcodmo_term
peak_5	Relative concentration of coral extract compound from peak 5 from the 254 nm spectra	unitless	no_bcodmo_term
peak_6	Relative concentration of coral extract compound from peak 6 from the 254 nm spectra	unitless	no_bcodmo_term
peak_7	Relative concentration of coral extract compound from peak 7 from the 254 nm spectra	unitless	no_bcodmo_term
peak_8	Relative concentration of coral extract compound from peak 8 from the 254 nm spectra	unitless	no_bcodmo_term
peak_9	Relative concentration of coral extract compound from peak 9 from the 254 nm spectra	unitless	no_bcodmo_term
peak_10	Relative concentration of coral extract compound from peak 10 from the 254 nm spectra	unitless	no_bcodmo_term
peak_11	Relative concentration of coral extract compound from peak 11 from the 254 nm spectra	unitless	no_bcodmo_term
peak_12	Relative concentration of coral extract compound from peak 12 from the 254 nm spectra	unitless	no_bcodmo_term

Database

Contribute Data

Dataset: Montipora chemical fingerprints
Deployment: Aeby_2014

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Montipora chemical fingerprintsDeployment: Aeby_2014

Dataset acquisition description

Dataset Processing Description

Dataset: Montipora chemical fingerprints
Deployment: Aeby_2014