Dataset: MWS accession numbers
Deployment: Aeby_2014

GenBank accession numbers for pathogens identified as causing MWS from Kaneohe bay, Oahu, Hawaii

Principal Investigator:

Greta Aeby (University of Hawaiʻi at Mānoa, SOEST)

Co-Principal Investigator:

Sean Callahan (University of Hawai'i, UH)

Evelyn Cox (University of Hawai'i, UH)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Host-environment-pathogen interactions in a model coral disease system (coral-pathogen interaction)

Current State:

Final no updates expected

Version:

2014-08-28

Version Date:

2014-08-28

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/545998/data

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Description

Related datasets:

Montipora antibacterial-aqueous
Montipora antibacterial-mucus
Montipora antibacterial-organic
Montipora chemical fingerprints
MWS lesion progression

Related references:

Ushijima et al. 2012: Vibrio owensii Induces the Tissue Loss Disease Montipora White Syndrome in the Hawaiian Reef Coral Montipora capitata. PLoS ONE 7: e46717.

Ushijima et al. 2013: Draft Genome Sequence of Vibrio coralliilyticus Strain OCN008, Isolated from Kāne'ohe Bay, Hawai'i. Genome Announcements. 1:e00786-13-e00786-13.

Ushijima et al. 2014: Vibrio coralliilyticus Strain OCN008 Is an Etiological Agent of Acute Montipora White Syndrome. Applied and Environmental Microbiology 80:2102-2109.

Methods & Sampling

Dataset acquisition description

See the publications above for detailed methodology.

For accession numbers JX127210 to JX127222 from Ushijima et al. 2012, PLoS ONE
For accession number AVOO00000000 from Ushijima et al. 2013, Genome announcements.
For accession numbers KF042020 to KF042038 from Ushijima et al. 2014, Applied and Environmental Microbiology.

Brief summary of methods:

Samples were collected from Kaneohe Bay, Oahu, Hawaii in 2011, 2012, and 2013.

Bacteria were isolated using standard microbiology techniques. DNA was extracted using a phenol-chloroform technique. PCR amplification was performed using DNA oligos. Refer to publications mentioned above for detailed methodology, statistical analyses, and results.

Data Processing Description

Dataset Processing Description

DNA sequences were trimmed using Gene Construction Kit software.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard or to match other datasets in project
- replaced blanks with underscores

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961814	NSF Division of Ocean Sciences

Instruments

None available yet

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Genbank_accession_num	GenBank accession number	unitless	accession_number
organism	organism taxonomic name and strain	unitless	taxon
description	description of sequenced genetic material	unitless	no_bcodmo_term
accession_link	link to GenBank accession number page	unitless	accession_number

Database

Contribute Data

Dataset: MWS accession numbers
Deployment: Aeby_2014

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: MWS accession numbersDeployment: Aeby_2014

Dataset acquisition description

Dataset Processing Description

Dataset: MWS accession numbers
Deployment: Aeby_2014