Dataset: A. pisonii: fecundity
Deployment: Griffen_mangroves

Determining fecundity of Aratus pisonii populations in mangrove and salt marsh habitats

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Current State:

Final no updates expected

Version Date:

2016-02-25

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/639234/data

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Description

This data is part of a project exploring the impact of climate-change induced range expansion on traditional ecogeographic patterns of life history. Data were collected June-August 2013.

Related Reference:
Riley ME, Griffen BD (2017) Habitat-specific differences alter traditional biogeographic patterns of life history in a climate-change induced range expansion. PLOS One 12(5):e0176263.

Methods & Sampling

Dataset acquisition description

To determine egg weight and fecundity, ovigerous females were collected from mangrove and salt marsh habitats and placed upon ice. Eggs were removed from the pleopods of each female, egg stage (eyed/noneyed) was determined, and crabs with recently extruded, noneyed eggs were dissected to ensure that they were post-vitellogenic (i.e. all eggs were extruded). A subset of eggs was taken from each individual and counted under a dissecting microscope. These subsets were dried at 65°C for 48 hours and weighed. We then determined the relationship between egg count and dry egg mass using a linear regression, and used the slope of this relationship (which indicates the average dry mass of a single egg) along with total brood dry weight to calculate total fecundity for individuals from both habitats.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- replaced spaces with underscores; removed commas
- added lat and lon for mapping purposes
- reordered columns

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1129166	NSF Division of Ocean Sciences

Instruments

Microscope - Optical

Supplied Name:

Supplied Description:

Dissecting microscope

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

scale

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
habitat	Dominant habitat: mangrove forest; marsh; mixed marsh	unitless	site_descrip
site	Site identification	unitless	site
lat	Latitude; north is positive	decimal degrees	lat
lon	Longitude; east is positive	decimal degrees	lon
crab	individual crab identification	unitless	sample
carap_width	Carapace width	millimeters	width
egg_stage	Egg stage: eyed or not eyed	unitless	stage
eggs_subsample	Number of eggs in subsample	eggs	count
eggs_wgt_subsample	Egg subsample weight (mg)	milligrams	weight
eggs_wgt_total	Total brood weight (g)	grams	weight
eggs_total_estimate	Total fecundity estimate (number of eggs)	eggs	count

Database

Contribute Data

Dataset: A. pisonii: fecundity
Deployment: Griffen_mangroves

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: A. pisonii: fecundityDeployment: Griffen_mangroves

Dataset acquisition description

Dataset Processing Description

Dataset: A. pisonii: fecundity
Deployment: Griffen_mangroves