Dataset: MEPS 2015: chemistry
Deployment: MCR_Edmunds

Chemical and physical measurements in coral tanks in Moorea and Okinawa

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Co-Principal Investigator:

Robert Carpenter (California State University Northridge, CSUN)

Student:

Nicolas R. Evensen (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

RUI: Ocean Acidification- Category 1- The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs (OA_Corals)

Current State:

Final no updates expected

Version:

2016-03-10

Version Date:

2016-03-10

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/640155/data

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Description

Two laboratory experiments were completed using Porites lutea and Montipora aequituberculata from back and fringing reef habitats. First, in Moorea, French Polynesia, we evaluated the efficacy of linear extension as a dependent variable characterizing the response of corals in competitive encounters, as well as a means to test for an effect of elevated pCO2 on linear extension. Second, at Sesoko Island, Okinawa, we applied the techniques developed in Moorea to test the effects of elevated pCO2 on the ability of corals to compete with one another for space.

Temperature, pH, pCO2, total alkalinity, and the aragonite saturation state (omega) were measured daily in the tanks.

Related Reference:
Evensen, Nicolas R., Peter J. Edmunds, Kazuhiko Sakai. Effects of pCO2 on spatial competition between the corals Montipora aequituberculata and Porites lutea. MEPS 541:123-134 (2015) doi:10.3354/meps11512.

Related Datasets:
MEPS 2015: field survey
MEPS 2015: linear extension

Methods & Sampling

Dataset acquisition description

The ecological relevance of interactions between M. aequituberculata and P. lutea lies in the common occurrence of these corals in shallow reef habitats, and the frequency with which they encounter one another, as observed during preliminary field observations. To quantify these effects, we evaluated coral community structure in the back reef of Moorea along the East and West shores that are sampled annually as part of the Moorea Coral Reef LTER (Edmunds 2013). Photoquadrats recorded in the back reef of these shores (at LTER sites 3 and 6) in 2010, and analyzed for coral cover using Coral Point Count [CPCe] software [Kohler & Gill 2006]), were used to quantify the abundance of Montipora and massive Porites. These images were also used for an additional analysis in which colonies of Porites and Montipora were scored for contact with one another. All colonies of Porites and Montipora were evaluated for contact, with contact scored when colonies were < 5 mm from one another, and these points of contact inferred to be sites of interspecific competition. The number of colonies of each taxon involved in interspecific competition was expressed as a percentage of the total number of colonies in each taxon that were present in all the photoquadrats evaluated. It was however not possible to determine the outcome (i.e., which colony was dominant versus subordinate) of most competitive encounters in the planar photographs.

For further details, see Evenson et al (2015)

Data Processing Description

Dataset Processing Description

Chemical conditions in the tanks were compared among tanks with a 2-way ANOVA, with treatment as a fixed effect, and tank a random factor nested in each treatment. The tank effect was dropped from the analyses when not significant at p = 0.250 (Quinn & Keough 2002). Competitive pairings in both experiments were used in a split-plot ANOVA with one between-plot effect (pCO2), one within-plot effect (type of pairing), and replicate tanks (treated as plots, with 3 in Moorea and 4 in Okinawa) nested in each pCO2 treatment. Treatment effects were assessed after 24 d in Moorea and after 21 d in Okinawa using linear extension as the dependent variable. Planned comparisons of differences between control and interspecific pairings in both the ambient and elevated CO2 treatments, for M. aequituberculata in Moorea and both corals in Okinawa, were selected to test explicitly for an effect of OA on the growth of corals engaged in interspecific competition. Planned comparisons were completed following Sokal & Rohlf (1995). The assumptions of normality and homosce - dasticity required for the ANOVAs were evaluated through graphical analyses of residuals. SYSTAT 11 running on a Windows operating system was used for all analyses.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- reformatted date from m/d/yyyy to yyyy-mm-dd
- added lat and lon columns

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1041270	NSF Division of Ocean Sciences
OCE-1236905	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name:

Supplied Description:

In Moorea, an automatic titrator (Model T50, Mettler-Toledo) was used.,br /> In Okinawa, an AT titration analyzer (Kimoto ATT-05).

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

pH Sensor

Supplied Name:

Supplied Description:

Orion, 3-stars mobile coupled with a Mettler DG 115-SC pH electrode

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Water Temperature Sensor

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
measure_type	seawater parameter: temperature pH pCO2 Total Alkalinity Aragonite saturation state	text	no_bcodmo_term
units	units for the seawater parameter measured	text	no_bcodmo_term
location	survey location	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
date	sampling date	yyyy-mm-dd	date
tank_1	measurement in tank 1	degrees Celsius	no_bcodmo_term
tank_2	measurement in tank 2	degrees Celsius	no_bcodmo_term
tank_3	measurement in tank 3	degrees Celsius	no_bcodmo_term
tank_4	measurement in tank 4	degrees Celsius	no_bcodmo_term
tank_5	measurement in tank 5	degrees Celsius	no_bcodmo_term
tank_6	measurement in tank 6	degrees Celsius	no_bcodmo_term
tank_7	measurement in tank 7	degrees Celsius	no_bcodmo_term
tank_8	measurement in tank 8	degrees Celsius	no_bcodmo_term
tank_9	measurement in tank 9	degrees Celsius	no_bcodmo_term

Database

Contribute Data

Dataset: MEPS 2015: chemistry
Deployment: MCR_Edmunds

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: MEPS 2015: chemistryDeployment: MCR_Edmunds

Dataset acquisition description

Dataset Processing Description

Dataset: MEPS 2015: chemistry
Deployment: MCR_Edmunds