Dataset: Carcinus: hemocyte density
Deployment: Griffen_lab

Counts of circulating hemocyte density in Carcinus maenas

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

Co-Principal Investigator:

April M. H. Blakeslee (Smithsonian Environmental Research Center, SERC)

Amy E. Fowler (Smithsonian Environmental Research Center, SERC)

Carolyn L. Keogh (University of Georgia, UGA)

Contact:

Carolyn L. Keogh (University of Georgia, UGA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Current State:

Final no updates expected

Version:

Version Date:

2016-04-08

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/642497/data

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Description

Sub-project: "Linking individual variation as a key to prediction."

Related Reference: Blakeslee, A.M., C.L. Keogh, A.E. Fowler, B. Griffen. 2015. Assessing the effects of trematode infection on invasive green crabs in eastern North America. PLOS One 10(6): e0128674.(pdf)

Related Dataset: Carcinus: parasites physiology behavior

Methods & Sampling

Dataset acquisition description

Work took place at Shoals Marine laboratory, Appledore Island, ME using Carcinus maenas collected from the Great Bay Estuary at University of New Hampshire Jackson Estuarine Lab at Adams Point, New Hampshire.

See Blakeslee et al (2015) PlosOne for complete methodology (pdf)

Data Processing Description

Dataset Processing Description

Mean hemocyte densities were calculated from the average counts of 6 squares on a KOVA hemocytometer slide, and are corrected for dilution of the hemolymph with anticoagulant to give an estimate of total hemocytes per uL of hemolymph.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- reformated date from m/d/yyyy to yyyy-mm-dd
- added site, lat, lon columns for mapping purposes

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1129166	NSF Division of Ocean Sciences

Instruments

Microscope - Optical

Supplied Name:

Supplied Description:

compound microscope

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
site	sampling location	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
crab	Crab identification number	unitless	sample
treatment	Treatment	unitless	treatment
time_exposure	Time exposed to parasite infection	HH:MM	time_elapsed
hemocytes_uL	average hemocytes per uL of hemolymph	Hem/uL	no_bcodmo_term

Database

Contribute Data

Dataset: Carcinus: hemocyte density
Deployment: Griffen_lab

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Carcinus: hemocyte densityDeployment: Griffen_lab

Dataset acquisition description

Dataset Processing Description

Dataset: Carcinus: hemocyte density
Deployment: Griffen_lab