Dataset: SEEPC larval collections: MOCNESS
Deployment: AT21-02

MOCNESS collections of invertebrate larvae throughout water column near methane seep sites: Barbados Accretionary Prism, 2012; Gulf of Mexico, 2014;Western Atlantic Margin, 2015 (SEEPC Project)

Principal Investigator:

Craig M. Young (University of Oregon, OIMB)

Co-Principal Investigator:

Svetlana A. Maslakova (University of Oregon, OIMB)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Connectivity in western Atlantic seep populations: Oceanographic and life-history processes underlying genetic structure (SEEPC)

Current State:

Final no updates expected

Deployment Synonyms:

SEEPC-Barbados

Version Date:

2016-09-30

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/659929/data

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Description

This dataset is a collection log showing the preliminary identification of larval types and the final disposition of individual larval specimens from MOCNESS plankton samples.

Methods & Sampling

Dataset acquisition description

One-meter MOCNESSS nets fitted with 300 um mesh nets were towed obliquely over methane seep sites, closing and opening nets at intervals that varied with the depth. Upon recovery, all samples were sorted completely over the next 12 hours or less, picking all individual invertebrate larvae from the samples, assigning morphotypes, photographing with a microscope, and preserving either for Scanning Electron Microscopy (glutaraldehyde, 2012 only) or for molecular genetics (95% ETOH).

Data Processing Description

Dataset Processing Description

2012: All larvae prepared for SEM were imaged. Larvae of some of the major groups (notably molluscs, bryozoans) were sequenced.

2014 and 2015: Larvae of some of the major groups (notably molluscs and bryozoans) were sequenced.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed some parameters to BCO-DMO standard
- replaced blanks, '?', '-' with 'nd' (no data)
- reformatted date from m/d/yyyy to yyyy-mm-dd
- removed 'm' from depth values
- converted lat and lon to decimal degrees
- removed or replaced special characters
- added cruise_id and year columns

Version: 2017-02-13 [added EN531 collection data]
Replaces version: 2016-09-30

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1030453	NSF Division of Ocean Sciences

Instruments

MOCNESS1

Supplied Name:

Supplied Description:

MOCNESS plankton system with 300 um mesh nets.

Instrument Type

Generic Name: MOCNESS1

Acronym: MOC1

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/NETT0097/

Generic Description:

The Multiple Opening/Closing Net and Environmental Sensing System or MOCNESS is a family of net systems based on the Tucker Trawl principle. The MOCNESS-1 carries nine 1-m2 nets usually of 335 micrometer mesh and is intended for use with the macrozooplankton. All nets are black to reduce contrast with the background. A motor/toggle release assembly is mounted on the top portion of the frame and stainless steel cables with swaged fittings are used to attach the net bar to the toggle release. A stepping motor in a pressure compensated case filled with oil turns the escapement crankshaft of the toggle release which sequentially releases the nets to an open then closed position on command from the surface. -- from the MOCNESS Operations Manual (1999 + 2003).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cruise identification	unitless	cruise_id
year	year of sampling	year	year
date	date of sampling formatted as yyyy-mm-dd	year-month-day	date
site	sampling site	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
depth_range	collection depth range	meters	depth_range
larva_id	unique number assigned to each individual larva	unitless	sample
larval_type	Abbreviation for the general larval form: Actinotroch - AC Amphioxus - AX Anthozoan larva AN Asteroid Juvenile - AJ Auricularia - AU Bipinnaria - BI Bivalve veliger - BV Brachiolaria - BR Brachiopods - BC Chaetopterid CH Cyprid - CP Chaetognath - CT Cyphonautes - CY Doliolaria - DO Echinopluteus - EC Entoprocts - EN Egg - EG Gastropod veliger - GV Glottidia - GL Gymnosome larva - GY Megalopa MG Mitraria - MI Mullers - MU Nectochaete - NE Pelagosphaera - PE Pentacula - PN Pilidium - PI Planulae - PL Planuliform - PA Planuliform palaeonemertean - PP Pteropod shell - PT Ophiopluteus - OP Ophiuroid juvenile - OJ Sponge larva - SL Tadpole - TA Trochophore - TR Tornaria - TO Unknown - UK Zoanthid - ZO Zoea - Z	unitless	taxon
morphotype	initial morphological classification; numbered sequentially within larval forms	unitless	taxon
fixation	method of preservation	unitless	no_bcodmo_term
comments	comments and notes	unitless	comment

Database

Contribute Data

Dataset: SEEPC larval collections: MOCNESS
Deployment: AT21-02

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: SEEPC larval collections: MOCNESSDeployment: AT21-02

Dataset acquisition description

Dataset Processing Description

Dataset: SEEPC larval collections: MOCNESS
Deployment: AT21-02