Dataset: Grazing experiment 2 and 3: daily cell counts
Deployment: Lab_Olson_B

The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.  Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts.  Cells were counted live on a BD FACSCalibur flow cytometer and were quantified based on side scatter and red fluorescence parameters.  Prior to counting, a measured volume of 2µm yellow-green latex bead solution (Flow Check intermediate intensity level 1 fluorescence in nanopure water) was added to each sample of E. huxleyi.  The bead solution was counted manually with epi-fluorescence microscopy using UV light.  Cell concentration was determined by the ratio between cells and beads counted.  The dilution volume was then calculated to achieve a cell density that would remain close to or under 100,000 cells per ml during the next day of growth.