Viability determined with CellTracker Green CMFDA (Life Technologies); specimens >63 µm. Experimental conditions inside Biospherix C-chambers were controlled with Biospherix ProO2 and ProCO2 sensors and controllers. These Biospherix systems have 2% precision.
Related References:
Bernhard, J.M., J.C. Wit, D. C. McCorkle (2015) Culturing fundamentals used to design and execute a long-term multi-stressor experiments to assess impact of ocean acidification, deoxygenation, and warming on benthic foraminiferal community composition, growth, and carbonate yield. 2015 American Geophysical Union Fall Meeting, San Francisco, abstract #PP52B-01.
Bernhard, J.M., J.C. Wit, D.C. McCorkle (2016) Anthropocene triple stress: Impact of experimentally induced ocean acidification, deoxygenation and warming on benthic foraminiferal community composition and growth. Geological Society of America Abstracts with Programs. Vol. 48, No. 7, doi: 10.1130/abs/2016AM-281509.