Dataset: Synechococcus Si uptake: silicic acid gradients
Deployment: Krause_DISL_2013-2015

Silica uptake by Synechococcus cells in response to a gradient of silicic acid concentrations

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/674295

Principal Investigator:

Jeffrey W. Krause (Dauphin Island Sea Lab, DISL)

Co-Principal Investigator:

Mark A. Brzezinski (University of California-Santa Barbara, UCSB)

Contact:

Jeffrey W. Krause (Dauphin Island Sea Lab, DISL)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Understanding the Role of Picocyanobacteria in the Marine Silicate Cycle (Si_in_Syn)

Current State:

Preliminary and in progress

Version:

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Description

Monocultures of four Synechococcus cyanobacterium clones of were used to examine the rate of Si accumulation in response to a gradient of silicic acid concentrations [Si(OH)4] . The data include the silicic acid concentration used during the experiment and the silica uptake rate. The experiments took place at the University of Santa Barbara Marine (UCSB) Science Institute between June and November of 2012 and the Dauphin Island Sea Laboratory (DISL) between March of 2013 and August of 2015.

Methods & Sampling

Dataset acquisition description

Clones (1333, 1334, 2370, 2515) were maintained in aged surface Sargasso Sea water with f/2 media constituents. For each of the four clones, Sargasso Seawater (ambient Si(OH)4 ~1 uM) was amended with 10 - 14 levels of silicic acid. Experiments examined the response of the rate of Si accumulation across a silicic acid concentration gradient from 1 to 50 uM (kinetic_type:low) or from 1 to 500 uM (kinetic_type: high). Only clone 1333 was used for the 1 to 500 uM silicic acid concentration experiment. The high kinetic type experiments took place at the UCSB Marine Science Institute and the low kinetic type experiments took place at DISL.

Each incubation was terminated by filtration and processed for measurement of 32Si activity following Krause et al. (2011). After aging for 120 days, secular equilibrium between 32Si and its daughter 32P was achieved and 32Si activity was determined using gas-flow proportional counting using GM 25-5 multicounters (Risø National Laboratory, Technical University of Denmark).

Full details of culturing and experimental methods are described in Brzezinski et al. (in review). (as of 05 Jan 2017)

Data Processing Description

Dataset Processing Description

No further processing.

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values replaced with no data value 'nd'
* latitude and longitude of Dauphin Island Sea Lab and UCSB Marine Institute added to dataset
* combined data for three separate high kinetic type experiments and added data column "exp_id" which is an identifier to distinguish between data from different experiments.
* combined "low" and "high" kinetic experiment data and created data column "kinetic_type" which has value (high|low)

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1335012	NSF Division of Ocean Sciences

Instruments

GM multicounter

Supplied Name: GM 25-5 multicounter

Supplied Description:

GM 25-5 multicounters (Risø National Laboratory, Technical University of Denmark)

Instrument Type

Generic Name: GM multicounter

Generic Description:

A gas flow multicounter (GM multicounter) is used for counting low-level beta doses. GM multicounters can be used for gas proportional counting of 32Si to 32P. For more information about GM multicounter usage see Krause et. al. 2011.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
kinetic_type	Kinetic type representing the gradient of silic acid concentrations used (high=1-500uM;low=1-50uM)	unitless	exp_type
exp_id	Experiment identifier	unitless	exp_id
clone_lat	Latitude of the clone collection site	decimal degrees	lat
clone_lon	Longitude of the clone collection site	decimal degrees	lon
clone_id	Synechococcus clone identifier (NCMA strain and CCMP number)	unitless	sample
silicic_acid	Silicic acid concentration [Si(OH)4]	micromolar (uM)	Silicate
uptake_rate	Silica uptake rate	reciprocal hours (h-1)	Si

Database

Contribute Data

Dataset: Synechococcus Si uptake: silicic acid gradients
Deployment: Krause_DISL_2013-2015

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Synechococcus Si uptake: silicic acid gradientsDeployment: Krause_DISL_2013-2015

Dataset acquisition description

Dataset Processing Description

Dataset: Synechococcus Si uptake: silicic acid gradients
Deployment: Krause_DISL_2013-2015