Dataset: [DEPRECATED] AT37-13 Colonization Experiment Isotopes
Deployment: AT37-13

[DEPRECATED] Averages and standard deviation of stable isotopes (d13C and d15N) across species for all macrofauna found on each substrate (carbonate rocks, woods, and bones) of the colonization experiment held in Mound 12

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/747645

Principal Investigator:

Lisa A. Levin (University of California-San Diego, UCSD-SIO)

Co-Principal Investigator:

Gregory Rouse (University of California-San Diego, UCSD-SIO)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative research: Quantifying the biological, chemical, and physical linkages between chemosynthetic communities and the surrounding deep sea (Costa Rica Seeps)

Current State:

Restricted

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Description

Status (2021-02-09): This dataset has been deprecated (obsoleted). It has been replaced by dataset https://www.bco-dmo.org/dataset/747575.

Averages and standard deviation of stable isotopes (d13C and d15N) across species for all macrofauna found on each substrate (carbonate rocks, woods, and bones) of the colonization experiment held in Mound 12. Substrates were collected using HOV Alvin on R/V Atlantis cruise AT37-13.

Methods & Sampling

Dataset acquisition description

Carbonate rocks, woods, and bones deployed for 7 years at Mound 12 across seepage gradients were collected using HOV Alvin, and washed in miliQ water onboard. Before preserving, the sample was kept cold and animals were picked to sample tissue for isotope analysis. The tissue (aprox. 0.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. The remaining washed sample was sieved through 0.3 mm mesh, separating the sample in two fractions (a fine fraction with the meiofauna, and a coarser one with the macrofauna), both preserved in 96% Ethanol. In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Stable isotopes measurements (d13C and d15N) were carried out using a Costech elemental analyzer coupled to a Micromass Iso-prime isotope ratio mass spectrometer (EA/IRMS) at Washington State University.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- modified parameter names.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1634172	NSF Division of Ocean Sciences

Instruments

Elemental Analyzer

Supplied Name: Costech elemental analyzer

Supplied Description:

Stable isotopes measurements (d13C and d15N) were carried out using a Costech elemental analyzer coupled to a Micromass Iso-prime isotope ratio mass spectrometer (EA/IRMS) at Washington State University.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: Micromass Iso-prime isotope ratio mass spectrometer

Supplied Description:

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
substrate	Type of substrate	unitless	site_descrip
alvin_dive	Number of the Alivn dive from which the sample was collected	unitless	dive_id
habitat	Seepage activity (active or inactive)	unitless	site_descrip
d13C_avg	Average of carbon isotope signature of animals on that substrate	per mil	d13C
d15N_avg	Average of nitrogen isotope signature of animals on that substrate	per mil	d15N
d13C_stdev	Standard deviation of carbon isotope signature of animals on that substrate	per mil	d13C
d15N_stdev	Standard deviation of nitrogen isotope signature of animals on that substrate	per mil	d15N

Database

Contribute Data

Dataset: [DEPRECATED] AT37-13 Colonization Experiment Isotopes
Deployment: AT37-13

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: [DEPRECATED] AT37-13 Colonization Experiment IsotopesDeployment: AT37-13

Dataset acquisition description

Dataset Processing Description

Dataset: [DEPRECATED] AT37-13 Colonization Experiment Isotopes
Deployment: AT37-13