Dataset: ELF field data
Deployment: AT39-05

NCBI accession numbers for raw RNA-seq fastq files derived from laboratory-maintained Trichodesmium erythraeum cultures

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/766128

Principal Investigator:

Eric A. Webb (University of Southern California, USC)

Co-Principal Investigator:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)

Version:

Deployment Synonyms:

TriCoLim

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Description

These data report enzyme-linked fluorescence (ELF) assays of Trichodesmium colonies collected from RV/Atlantis cruise AT39-05, Feb-Mar 2018.

Methods & Sampling

Dataset acquisition description

Methods are described in Dyhrman et al (2002).

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- re-formatted date from m/d/yyyy to yyyy-mm-dd
- re-formatted date to 24 hour time

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1657757	NSF Division of Ocean Sciences
OCE-1657766	NSF Division of Ocean Sciences

Instruments

Microscope - Optical

Supplied Name: ZeissAxioplan2 microscope

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
station	station identifier	unitless	station
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
date_local	local sampling date formatted as yyyy-mm-dd	unitless	date_local
time_local	local sampling time formatted as HH:MM	unitless	time_local
slide_label	slide label	unitless	sample
colony_description	Whether Trichodesmium colony morphology is Puff/Tuft/Raft	unitless	sample_descrip
Tricho_filament_thickness	Filament Thickness (e.g. normal; thick; some normal some thick)	unitless	sample_descrip
Tricho_filament_fluor	Trichodesmium filament fluorescence pigmentation on DAPI LP filter (orange; reddish; greenish)	unitless	sample_descrip
Tricho_filament_ELF_glow	Filament ELF Glow (e.g. none/few/some/majority lightly/moderately speckled	unitless	sample_descrip
ELF_reaction_results_plus_minus	Plus or Minus	unitless	sample_descrip
backgrnd_speckling	background speckling (e.g. none; little; some; a lot)	unitless	sample_descrip
backgrnd_speckling_filamaments	speckling around filaments (e.g. none; little; some; a lot)	unitless	sample_descrip
backgrnd_speckling_colonies	speckling around colonies (e.g. none; little; some; a lot - n/a if no colony)	unitless	sample_descrip

Database

Contribute Data

Dataset: ELF field data
Deployment: AT39-05

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: ELF field dataDeployment: AT39-05

Dataset acquisition description

Dataset Processing Description

Dataset: ELF field data
Deployment: AT39-05