Dataset: Pigments HPLC
Deployment: NBP1801

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/778881

Principal Investigator:

Giacomo DiTullio (College of Charleston, CofC)

Co-Principal Investigator:

Peter Lee (College of Charleston, CofC)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay (CICLOPS)

Version:

Deployment Synonyms:

CICLOPS, Cobalamin and Iron Co-Limitation Of Phytoplankton Specie

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Description

Algal pigment concentrations as measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018.

Methods & Sampling

Dataset acquisition description

Algal HPLC samples were collected by gentile filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002).

High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors

Data Processing Description

Dataset Processing Description

Pigment concentrations were determined using standard peak integration procedures with Agilent’s ChemStation (version B.03.02), and entered into Microsoft Excel Spreadsheets for submission to BCO-DMO. Parameters reported were: chlorophyll c3, chlorophyllide, magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester, chlorophyll c2, chlorophyll c1, peridinin, pheophorbide a, 19-prime butanoyloxyfucoxanthin, fucoxanthin, neoxanthin, prasinoxanthin, violaxanthin, 19-prime hexanoyloxyfucoxanthin, diadinoxanthin, cis-fucoxanthin, alloxanthin, diatoxanthin, monadoxanthin, zeaxanthin, lutein, crocoxanthin, chlorophyll b, divinyl chlorophyll a, chlorophyll a, pheophytin a, carotene-alpha and carotene-beta.

BCO-DMO processing notes:

Adjusted column names
Adjusted date format to yyyy-mm-dd for increased interoperability
Version 2: An updated calibration curve was used to recalculate the chlorophyll a concentrations

More information about this dataset deployment

Funding

Award Number	Funding Source
OPP-1644073	NSF Office of Polar Programs (formerly NSF PLR)

Instruments

High-Performance Liquid Chromatograph

Supplied Name: Agilent 1100

Supplied Description:

High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date (UTC) - format: yyyy-mm-dd	unitless	date
Station	Station Identifier	unitless	station
Latitude	Latitude (South is negative)	decimal degrees	lat
Longitude	Longitude (West is negative)	decimal degrees	lon
Depth	Sample depth	meters (m)	depth
Niskin	Niskin Bottle Number	unitless	bot_Nis
Sample	Sample Number	unitless	sample
Fltr_Vol	Volume Filtered	liter (L)	vol_filt
Chl_C3	Chlorophyll c3	nanogram per liter (ng/L)	chl_c3
Chl_lide	Chlorophyllide	nanogram per liter (ng/L)	chlide
MgDVP	Magnesium-2;4-divinyl	nanogram per liter (ng/L)	no_bcodmo_term
Chl_C2	Chlorophyll c2	nanogram per liter (ng/L)	chl_c2
Chl_C1	Chlorophyll c1	nanogram per liter (ng/L)	chl_c1
Peridinin	Peridinin	nanogram per liter (ng/L)	peridinin
Ph_ide	Pheophorbide a	nanogram per liter (ng/L)	p_phorbide
But_19	19'-butanoyloxyfucoxanthin	nanogram per liter (ng/L)	fucox_but
Fuco	Fucoxanthin	nanogram per liter (ng/L)	fucox
Neo	Neoxanthin	nanogram per liter (ng/L)	neox
Prasino	Prasinoxanthin	nanogram per liter (ng/L)	prasinox
Viola	Violaxanthin	nanogram per liter (ng/L)	violax
Hex_19	19'-hexanoyloxyfucoxanthin	nanogram per liter (ng/L)	fucox_hex
Diadino	Diadinoxanthin	nanogram per liter (ng/L)	diadinox
cis_fuco	cis-Fucoxanthin	nanogram per liter (ng/L)	no_bcodmo_term
Allo	Alloxanthin	nanogram per liter (ng/L)	allox
Diato	Diatoxanthin	nanogram per liter (ng/L)	diatox
Monad	Monadoxanthin	nanogram per liter (ng/L)	monad
Zea	Zeaxanthin	nanogram per liter (ng/L)	zeax
Lutein	Lutein	nanogram per liter (ng/L)	lutein
Croco	Crocoxanthin	nanogram per liter (ng/L)	crocox
Chl_b	Chlorophyll b	nanogram per liter (ng/L)	chl_b
Chl_a_allomer	Chlorophyll a allomer	nanogram per liter (ng/L)	chl_a_allo
Chl_C2_MGDG	Chlorophyll c2 MGDG	nanogram per liter (ng/L)	no_bcodmo_term
DV_Chl_a	Divinyl chlorophyll a	nanogram per liter (ng/L)	chl_a2
Chl_a	Chlorophyll a	nanogram per liter (ng/L)	chl_a
Ph_tin	Phaeophytin a	nanogram per liter (ng/L)	p_phytin
a_Car	Alpha-carotene	nanogram per liter (ng/L)	carotene_a
b_Car	Beta-carotene	nanogram per liter (ng/L)	carotene_b

Database

Contribute Data

Dataset: Pigments HPLC
Deployment: NBP1801

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Pigments HPLCDeployment: NBP1801

Dataset acquisition description

Dataset Processing Description

Dataset: Pigments HPLC
Deployment: NBP1801