Cell abundances: Cell abundances were analyzed using flow cytometry as previously described (Van Oostende et al. 2017). Samples were collected in 5 mL-cryovials from 30-L Niksin bottles and fixed with 0.1% glutaraldehyde and frozen at -80C until later analysis in the shore based laboratory.
Ammonium: Ammonium concentration was measured manually fluorometrically using standard methods (Holmes et al 1999). Water was collected using Niskin sampling bottles. Samples were measured immediately upon retrieval and were not filtered prior to analysis. Five ml volumes were analyzed.
Nitrite: Nitrite concentration was measured manually colorimetrically using standard methods (Strickland and Parsons 1972). Water was collected using Niskin sampling bottles. Samples were measured immediately upon retrieval and were not filtered prior to analysis. Five ml volumes were analyzed.
Nitrite + Nitrate: Nitrite + Nitrate (NOx) concentration was measured using the chemiluminescence method (Garside 1982)
Water was collected using Niskin sampling bottles. Water was dispensed into 12-ml detainer vials and used in incubation experiments. Incubations were terminated by addition of saturated ZnCl2 and returned to the shore based laboratory. After mass spec analysis of the N2 gas in the vials, they were subsampled for analysis of total NOx in solution.
BCO-DMO Processing Notes:
- Combined NH4, NO2, Nitrate+Nitrate and flow cytometer datasets
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- combined the degrees and minutes columns of lat and long values to create lat and lon columns in decimal degrees, rounded columns to 6 digits.