Dataset: Suspended particles from in situ pumps
Deployment: KM1910

Suspended particles from in situ pumps from cruise KM1910

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/853048

Principal Investigator:

Matthew J. Church (University of Montana)

Co-Principal Investigator:

Sara Ferrón (University of Hawaiʻi at Mānoa, SOEST)

Erica Goetze (University of Hawaiʻi at Mānoa, SOEST)

Angelicque E. White (University of Hawaiʻi at Mānoa, SOEST)

Scientist:

Erin E. Black (University of Rochester)

Ashley Maloney (Princeton University)

Adam V. Subhas (Woods Hole Oceanographic Institution, WHOI)

Student:

Jennifer Kenyon (Massachusetts Institute of Technology, MIT)

Contact:

Ashley Maloney (Princeton University)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers (Chief Sci KM1910)

Version:

Deployment Synonyms:

EAGER, Chief Sci cruise, 2019 Chief Scientist Training Cruise

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Description

Methods & Sampling

Dataset acquisition description

Sampling and analytical procedures:

Particulates were collected using in situ pumps (25, 45, 75, 100, 125, 150, 225, and 300 m ocean depth) aboard the RV Kilo Moana. Pump heads were loaded with an acid-washed 51um nitex mesh screen as a pre-filter followed by a combusted (4.5hrs 450 degrees C) 142mm GF75 (Advantec nominal pore size 0.3um). An adjacent pump head was loaded with a 52um Sefar filter. Additional 142mm GF75 filters were placed in cages and secured to the side of pumps during deployments and served as wet blanks for PC, POC, PIC, PN analysis. Filters were subsampled with a metal cork borer of known area, folded (active side in), and stored in clean (combusted) foil at -20 degrees C until analysis. The 0.3 to 51um size fraction samples analyzed at Princeton (subsample IDs “m1” and “m2”) and MBL labs (subsample ID “z”) were not acidified so PC and d13C represent total C; subsamples analyzed at WHOI for POC were acidified (subsample IDs “q” (small size fraction 0.3 to 51um) and “r” (large size fraction >51um). Subsamples were oven dried overnight at 55-60 degrees C then packed in 9x11 tin capsules for analysis on EA IRMS. Additional subsamples were analyzed for PIC at WHOI (subsample IDs “p” (small size fraction 0.3 to 52um) and “y” (large size fraction >52um)). PIC samples were stored frozen (-20C) on the ship and dried at 60C for 6-8h on shore, stored in 47mm petri dishes. Filters were analyzed roughly 2 months after collection following established procedures (Dong et al., 2019, Subhas et al., 2019).

Problem report:

Large particle (>51 micron) PIC data was not collected at 100m because that filter was allocated for other measurements. Mass of N on subsample was too low for 15N measurement at 225 m and 300 m. Filter subsample “z” was only collected at one depth (25 m).

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:

- converted date_HST to YYYY-MM-DD format;
- created UTC date/time field in ISO8601 format.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1911831	NSF Division of Ocean Sciences
OCE-1911990	NSF Division of Ocean Sciences

Instruments

CHN Elemental Analyzer

Supplied Name: CHN flash analyzer at the WHOI Nutrient Facility

Supplied Description:

POC and PON were acid fumed with concentrated HCl and then POC and PON were measured by combustion via a Flash AE1112 Carbon/Nitrogen Analyzer using a Dynamic Flash Combustion technique. Certified reference material Acetanilide (Certificate#293514) is combusted with every run and an average recovery is provided with the sample results.

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

CO2 Analyzer

Supplied Name: Picarro 2101i

Supplied Description:

PIC samples were analyzed on a Picarro 2101i cavity ring-down CO2 isotope analyzer system with an AutoMate prep device front end. Detailed instrument methods are established and published in Dong et al., 2019 and Subhas et al., 2019. Samples were acidified manually in 12mL exetainers and left to sit for 1h to dissolve all PIC before mounting in the autosampler rack. Standards were run before, during, and after the samples in the same analytical session to calculate PIC concentration, using a well-characterized pure calcite standard of known isotopic composition (Iceland Spar). Calibrated PIC quantities (in micromoles) were blank-corrected using the mean value of McLane pump dipped blank filters. Blank-corrected quantities were then normalized to the volume pumped of each filter head to arrive at a PIC concentration at each pump depth in units of umol/L.

Instrument Type

Generic Name: CO2 Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/382/

Generic Description:

Measures atmospheric carbon dioxide (CO2) concentration.

Elemental Analyzer

Supplied Name: Vario ISOTOPE select (Elementar Isoprime)

Supplied Description:

A Vario ISOTOPE select (Elementar Isoprime) was used to quantify total C, N, and d13C and d15N. Samples are combusted/reduced at high temperature to create gases (CO2 and N2); column chromatography separates the gases; a thermal conductivity detector determines the carbon and nitrogen concentrations, followed by an isotope ratio mass spectrometer for bulk isotope composition. Measurements were calibrated with an in-house aminocaproic acid standard (ACROS) and four USGS standards (#41, #41a, #65, #25). Analytical uncertainty (determined from standards with natural abundance isotope values) was 0.2‰ for delta-15N and 0.1‰ for delta-13C.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: Europa 20-20

Supplied Description:

EA-IRMS at MBL Stable Isotope Lab: Europa 20-20 continuous-flow isotope ratio mass spectrometer interfaced with a Europa ANCA-SL elemental analyzer. Measurements were calibrated using international and in-house reference materials analyzed in the same analytical run. The analytical precision based on replicate analyses of isotopically homogeneous international standards is +/- 0.1 ‰ (1 sigma) for both δ 15N and δ 13C measurements, and about 1% relative on the %N and %C measurements.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

McLane Pump

Supplied Name: WTS 6-1-142LV

Supplied Description:

Samples were collected via two dual-filter head McLane large-volume in-situ pumping systems (WTS 6-1-142LV).

Instrument Type

Generic Name: McLane Pump

Generic Description:

McLane pumps sample large volumes of seawater at depth. They are attached to a wire and lowered to different depths in the ocean. As the water is pumped through the filter, particles suspended in the ocean are collected on the filters. The pumps are then retrieved and the contents of the filters are analyzed in a lab.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	Cruise identifier	unitless	cruise_id
sta	Station number	unitless	station
cast	Pump cast number	unitless	cast
lon	Longitude (degrees E)	decimal degrees	lon
lat	Latitude (degrees N)	decimal degrees	lat
deploy_ISO_DateTime_UTC	ISO datetime of pump deployment (UTC)	unitless	ISO_DateTime_UTC
date_deploy_HST	Local date (HST) of pump deployment	unitless	date_local
time_deploy_HST	Local time (HST) of pump deployment	unitless	time_local
recover_ISO_DateTime_UTC	ISO datetime of pump recovery (UTC)	unitless	ISO_DateTime_UTC
date_recover_HST	Local date (HST) of pump recovery	unitless	date_local
time_recover_HST	Local time (HST) of pump recovery	unitless	time_local
depth_m	Target depth of sample in meters	meters (m)	depth
PC_m1	Total Particulate Carbon (PC) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton; measurements corrected for 8 blank (wet) filters) subsample id "m1"	micromole per litre (umol/L)	TPC
PC_m2	Total Particulate Carbon (PC) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton; measurements corrected for 8 blank (wet) filters) subsample id "m2"	micromole per litre (umol/L)	TPC
PC_z	Total Particulate Carbon (PC) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at MBL; measurements corrected for 8 blank (wet) filters) sample id "z"	micromole per litre (umol/L)	TPC
P13C_m1	Delta-13C of total PC (permil vs. VPDB) analytical uncertainty 0.1 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton) subsample id "m1"	permil vs VPDB	d13C
P13C_m2	Delta-13C of total PC (permil vs. VPDB) analytical uncertainty 0.1 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton) subsample id "m2"	permil vs VPDB	d13C
P13C_z	Delta-13C of total PC (permil vs. VPDB) analytical uncertainty 0.1 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at MBL) sample id "z"	permil vs VPDB	d13C
PN_m1	Particulate Nitrogen (PN) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton; measurements corrected for 8 blank (wet) filters) subsample id "m1"	micromole per litre (umol/L)	N
PN_m2	Particulate Nitrogen (PN) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton; measurements corrected for 8 blank (wet) filters) subsample id "m2"	micromole per litre (umol/L)	N
PN_z	Particulate Nitrogen (PN) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at MBL; measurements corrected for 8 blank (wet) filters) sample id "z"	micromole per litre (umol/L)	N
P15N_m1	Delta-15N of PN (permil vs. VPDB) analytical uncertainty 0.2 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton) subsample id "m1"	permil vs. air-N2	d15N
P15N_m2	Delta-15N of PN (permil vs. VPDB) analytical uncertainty 0.2 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at Princeton) subsample id "m2"	permil vs. air-N2	d15N
P15N_z	Delta-15N of PN (permil vs. VPDB) analytical uncertainty 0.2 permil (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters not acidified before analysis at MBL) sample id "z"	permil vs. air-N2	d15N
SP_PIC_p	Small Particle Particulate Inorganic Carbon (PIC) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um), analyzed at WHOI) subsample id "p"	micromole per litre (umol/L)	PIC
LP_PIC_y	Large Particle Particulate Inorganic Carbon (PIC) [umol/L] from 52um Sefar mesh screen, analyzed at WHOI, subsample id "y"	micromole per litre (umol/L)	PIC
SP_POC_q	Small Particle Particulate Organic Carbon (POC) [umol/L] (prefiltered with 51um mesh screen onto combusted 142mm GF75 (nominal pore size 0.3um); filters acidified before analysis at WHOI) subsample id "q"	micromole per litre (umol/L)	POC
LP_POC_r	Large Particle Particulate Organic Carbon (POC) [umol/L] from 51um Nitex mesh screen, washed onto 25mm QMA; filters acidified before analysis at WHOI, sample id "r"	micromole per litre (umol/L)	POC

Database

Contribute Data

Dataset: Suspended particles from in situ pumps
Deployment: KM1910

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Suspended particles from in situ pumpsDeployment: KM1910

Dataset acquisition description

Dataset Processing Description

Dataset: Suspended particles from in situ pumps
Deployment: KM1910