Dataset: SCCS Bulk N2 Fixation Measurements
Deployment: SP1727

Bulk N2 fixation measurements

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/881060

Principal Investigator:

Jonathan P. Zehr (University of California-Santa Cruz, UCSC)

Co-Principal Investigator:

Kevin R. Arrigo (Stanford University)

Contact:

Kendra Turk-Kubo (University of California-Santa Cruz, UCSC)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Biogeochemical significance of the abundant, uncultivated symbiotic cyanobacteria UCYN-A (BSUCS)

Version:

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Description

These data were published in Turk-Kubo et al. (2021). Table 1, Figure 6, Table S6, Table S7.

Methods & Sampling

Dataset acquisition description

Samples were collected using standard oceanographic techniques. A CTD Rosette with 24 10L Niskin bottles was lowered to the maximum sampling depth and then brought back to the surface. Seawater was sampled directly from Niskin® bottles into acid-washed 1.2 l polycarbonate bottles through 210 µm Nitex® mesh (Wildco, Yulee, FL) to remove large grazers.

Methodology described in depth in Turk-Kubo et al. (2021)

Data Processing Description

Dataset Processing Description

Incubation bottles received 100 ml of 15N2-enriched seawater. 15N2-enriched seawater was generated and atom% enrichment was measured according to procedures described in detail by Mills et al. 2020. The 15N2-enriched seawater atom% enrichment ranged from 2.0–6.1% for SP1714 and 5.1–24.7% for SP1727. Bottles were incubated (24 h) under simulated in situ light using neutral density screening and maintained at surface seawater temperatures in flow-through on-deck incubators. Samples for atom% 15N of the ambient particulate matter were taken from corresponding depths at T0. At the termination of the incubation, samples for the analysis of 15N enrichment into particulate organic matter (ca. 1000 ml) were processed and measured, and NFRs were calculated. Limits of detection (LOD) and minimum quantifiable rates (MQRs) were calculated as in Gradoville et al. 2017

Methodology described in depth in Turk-Kubo et al. (2021)

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1559152	NSF Division of Ocean Sciences
OCE-1559165	NSF Division of Ocean Sciences

Instruments

Elemental Analyzer

Supplied Name: Elemental Combustion System (Costech Analytical Technologies)

Supplied Description:

Samples for bulk PON/POC and N2 fixation and CO2 fixation rates were measured on an Elemental Combustion System (Costech Analytical Technologies) interfaced to a Thermo Finnigan Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific) at the SOEST Biogeochemical Stable Isotope Facility at the University of Hawai’i, Manoa.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: hermo Finnigan Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific)

Supplied Description:

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	Cruise identifier	unitless	cruise_id
Station	Station number	unitless	station
Latitude	Sampling latitude, south is negative	decimal degrees	lat
Longitude	Sampling longitude, west is negative	decinal degrees	lon
Depth	Sample depth	meters (m)	depth
Average_NFR	Average nitrogen fixation rate	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
Stdev_NFR	Standard deviation nitrogen fixation rate	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
LOD	Limit of detection	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
MQR	Minimum quantifiable rate	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
Rep1_NFR	Nitrogen fixation rate, replicate 1	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
Rep2_NFR	Nitrogen fixation rate, replicate 2	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
Rep3_NFR	Nitrogen fixation rate, replicate 3	nanomoles nitrogen per liter per day (nmol N L-1 d–1)	N2_fix
Rep1_T	Incubation time, replicate 1	days	time_inc
Rep1_APN	Atom percentage nitrogen, replicate 1. T=F (%) [Time=final]	percentage (%)	pcent_composition
Rep1_PN	Particulate nitrogen, replicate 1	micromoles nitrogen per liter (µmol N L-1)	N
Rep2_T	Incubation time, replicate 2	days	time_inc
Rep2_APN	Atom percentage nitrogen, replicate 1. T=F (%) [Time=final]	percentage (%)	pcent_composition
Rep2_PN	Particulate nitrogen, replicate 2	micromoles nitrogen per liter (µmol N L-1)	N
Rep3_T	Incubation time, replicate 3	days	time_inc
Rep3_APN	Atom percentage nitrogen, replicate 1. T=F (%) [Time=final]	percentage (%)	pcent_composition
Rep3_PN	Particulate nitrogen, replicate 3	micromoles nitrogen per liter (µmol N L-1)	N
AtomPerc_N2_rep1	atom % enrichment in the 15N2 dissolved seawater	percentage (%)	pcent_composition
AtomPerc_PN_rep1	atom % enrichment in the particulate nitrogen	percentage (%)	pcent_composition

Database

Contribute Data

Dataset: SCCS Bulk N2 Fixation Measurements
Deployment: SP1727

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: SCCS Bulk N2 Fixation MeasurementsDeployment: SP1727

Dataset acquisition description

Dataset Processing Description

Dataset: SCCS Bulk N2 Fixation Measurements
Deployment: SP1727