Dataset: CliOMZ AT50-10 McLane log
Deployment: AT50-10

McLane pump logs - AUV Clio cruise

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/921847

Principal Investigator:

Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)

Co-Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

Contact:

Paloma Z. Lopez (Woods Hole Oceanographic Institution, WHOI)

Technician:

Paloma Z. Lopez (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories (CliOMZ)

Version:

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Description

Methods & Sampling

Dataset acquisition description

Seven Large Volume Water Transfer Systems (McLane pumps) were deployed during research cruise AT50-10 attached to a synthetic wire using a MASH2K winch and a dual filter sampler system. Deployments lasted 3 or 4 hours and seawater was filtered at specific depths. A combination of 0.2 um Supor membranes and 51 um pore size Nitex mesh was used for one side while the other side pump water through a Glass Fiber Filter (GFF). See data log for specific size fraction combination as well as specific samples taken.

Data Processing Description

Dataset Processing Description

The depths for McLane pumps (which lack pressure sensors) were calculated based on winch payout and a calibration curve generated aboard ship. The need for calibration arises from the MASH2K winch not being used with metallic wire for this application. By using (non-conducting) synthetic wire for trace metal clean needs, the wire wraps are not predictably uniform as with metallic wire. As a result and the MASH2K instrument borrowed from the East Coast Winch Pool was not programmed for depths using synthetic wire. To compensate for this the McLane pump payout calibration was estimated using the Trace Metal rosette deployed with a SSSG Pinger, with depth from the ship and the winch payout recorded at 17 intervals between 0 and 4287 meters. A power law relationship was calculated from this relationship by Tristan Horner where: True Payout = Raw Payout / ( 1.34 x Raw Payout^-0.026 ). This equation had a mean absolute standard deviation of 5m for the 17 depths recorded, with depth variance increasing with depth. This equation was applied to the payout readings from the winch when used for McLane pumps to calculate the deployment Depth (m) parameter.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1924554	NSF Division of Ocean Sciences

Instruments

McLane Large Volume Pumping System WTS-LV

Supplied Name: Large Volume Water Transfer Systems (McLane pumps)

Supplied Description:

Seven Large Volume Water Transfer Systems (McLane pumps; McLane labs: https://mclanelabs.com/wts-lv-large-volume-pump/) were deployed during research cruise AT50-10 attached to a synthetic wire using a MASH2K winch and a dual filter sampler system. A combination of 0.2 um Supor membranes and 51 um pore size Nitex mesh was used for one side while the other side pump water through a Glass Fiber Filter (GFF).

Instrument Type

Generic Name: McLane Large Volume Pumping System WTS-LV

Acronym: WTS-LV

Generic Description:

The WTS-LV is a Water Transfer System (WTS) Large Volume (LV) pumping instrument designed and manufactured by McLane Research Labs (Falmouth, MA, USA). It is a large-volume, single-event sampler that collects suspended and dissolved particulate samples in situ.

Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.

The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise_ID	Cruise ID AT50-10	unitless	cruise_id
Start_date_UTC	Pump start date	unitless	start_date
Start_time_UTC	Pump start time	unitless	time_begin
End_date_UTC	Pump end date	unitless	end_date
End_time_UTC	Pump end time	unitless	time_end
Station_number	Station number	unitless	station
Start_Lat	Pump start latitude	decimal degrees	lat_start
Start_Long	Pump start longitude	decimal degrees	lon_start
End_Lat	Pump end latitude	decimal degrees	lat_end
End_Long	Pump end longitude	decimal degrees	lon_end
Cast_number	Cast number	unitless	cast
Pump_number	Pump number	unitless	sample
Head_number_GFF_port	Pump head identification number at GFF water port in dual samlpler system	unitless	sample
Head_number_Supor_port	Pump head identification number at Supor/Nitex water port in dual samlpler system	unitless	sample
Winch_payout_reading	Winch payout reading	meters (m)	wire_out
Corrected_winch_payout	Correct winch payout	meters (m)	wire_out
Depth	Sample depth	meters (m)	depth
Total_time_filtered	Total time filtered	minutes (min)	time_sample
GFF_side_flow_meter_volume	Volume of water filtered through the GFF side in dual sampler system	liters (L)	vol_filt
Supor_side_flow_meter_volume	Volume of water filtered through the Supor side in dual sampler system	liters (L)	vol_filt
Filter_diameter	Filter diameter	millimeters (mm)	diameter
Comments_deployment	Comments on deployment	unitless	comment
SAMPLE_PRFEIX	Sample prefix	unitless	sample
SUPOR_0_2_FRACTION_PROTEIN1	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for global metaproteomic analysis	unitless	sample
SUPOR_0_2_FRACTION_DNA1	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for genomic DNA analysis, sample 1 of 2	unitless	sample
SUPOR_0_2_FRACTION_DNA2	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for genomic DNA analysis, sample 2 of 2	unitless	sample
SUPOR_0_2 FRACTION_eDNA	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for environmental DNA analysis	unitless	sample
SUPOR_0_2_FRACTION_pMetal	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for particulate metal content analysis	unitless	sample
SUPOR_0_2 FRACTION_POS	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for particulate organic sulfur analysis	unitless	sample
SUPOR_0_2_FRACTION_metatranscriptomics_Intercomparison	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for metatranscriptomic intercomparison analysis	unitless	sample
SUPOR_0_2_FRACTION_metagenomics_Intercomparison	Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for metagenomic intercomparison analysis	unitless	sample
NITEX_51_FRACTION_PROTEIN1	Fraction of 142mm diameter 51um pore size Nitex filter assigned for global metaproteomics	unitless	sample
NITEX_51_FRACTION_DNA1	Fraction of 142mm diameter 51um pore size Nitex filter assigned for genomic DNA analysis	unitless	sample
NITEX_51_FRACTION_pMetal	Fraction of 142mm diameter 51um pore size Nitex filter assigned for particulate metal content analysis	unitless	sample
NITEX_51_FRACTION_CULTURING	Fraction of 142mm diameter 51um pore size Nitex filter assigned for bacteria culturing	unitless	sample
GFF_FRACTION_PROTEIN1	Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for global metaproteomics	unitless	sample
GFF_FRACTION_CA	Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for carbonic anhydrase activity analysis	unitless	sample
GFF_FRACTION_PIGS	Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for photosynthetic pigments analysis	unitless	sample
GFF_FRACTION_POC	Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for particulate organic carbon analysis	unitless	sample
GFF_FRACTION_HG	Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for mercury content analysis	unitless	sample
SAMPLE_COMMENT	Comments on sample taken	unitless	comment

Database

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Dataset: CliOMZ AT50-10 McLane log
Deployment: AT50-10

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: CliOMZ AT50-10 McLane logDeployment: AT50-10

Dataset acquisition description

Dataset Processing Description

Dataset: CliOMZ AT50-10 McLane log
Deployment: AT50-10