Description:
For each pCO2 treatment there are three types of samples;
After de-lithing: These samples were taken immediately after the coccolithophores were acidified to dissolve their coccoliths and then neutralized to return the culture pH to the original pH. These samples were taken before the 24 h incubation period and therefore do not have a Light or Dark treatment.
Replicate: These represent the three replicates for each light condition and each CO2 treatment. These samples were taken after the cells incubated in either light or dark conditions for 24 h.
Blank: These samples were killed by the addition of formalin but were allowed to incubate in either light or dark conditions for 24 h.