Description:
The method used to identify the ASV as belonging to Pseudo-nitzschia sp. QIIME2 naiveBayes refers to the scikit-learn na?ve Bayes machine learning classifier (Pedregosa et al 2011) at default settings in QIIME2 (Bolyen et al 2019) using the curated database of existing Pseudo-nitzschia sequences in NCBI in Table S2 of Sterling et al. Megablast refers to using BLAST+ version 2.9 with the nucleotide (nt) database and manually examining the identification of ASVs with query coverage > 75% to known Pseudo-nitzschia sequences.