Dataset: DMSP-dependent_protein_structures

See Schuller et al. 2012 for detailed methods, which are paraphrased below:
"Expression and isolation of the dimethylsulfoniopropionate-dependent demethylase (DmdA) from recombinant E. coli that expressed the P. ubique DmdA (SAR11_0246) gene was performed as previously described in Reisch et al. (2008). Crystal screening of DmdA was performed in an anaerobic chamber consisting of an atmosphere of 95% nitrogen and 5% hydrogen using the microbatch diffusion technique and an IMPAX I-5 (Douglas Instruments, East Garston, United Kingdom) crystallization robot.

In addition to the native crystals, data was also collected on crystals that had been treated with either the substrate (2 mM DMSP) or the cofactor, tetrahydrofolate (2 mM THF). In both cases a solution of mother liquor was prepared, under anaerobic conditions,containing either DMSP or THF.

All data sets were collected at the Advanced Photon SER-CAT on beamline 22-ID (http://www.ser.aps.anl.gov/). Several programs from the CCP4 program suite (CCP4 website) were employed during structure determination and refinement. Various molecular replacement attempts were made using the program PHASER."

References:
Reisch CR, Stoudemayer MJ, Varaljay VA, Amster IJ, Moran MA, Whitman WB (2011). Novel pathway for assimilation of dimethylsulphoniopropionate widespread in marine bacteria. Nature 473:208–211. doi:10.1038/nature10078