Dataset: HOE-DYLAN cruises - Urea

Name: Urea concentrations from multiple HOE-DYLAN cruises, R/V Kilo Moana KM1215, KM1217, KM1219, at Station ALOHA from July to September 2012 (C-MORE project)
Published: 2023-08-10
Keywords: urea, DON, HOE-DYLAN, C-MORE, station ALOHA, LWCC, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.4055.1

Spatial Extent: N:22.817 E:-158.027 S:22.805 W:-158.05

Temporal Extent: 2012-07-09 - 2012-09-05

Project:

Center for Microbial Oceanography: Research and Education (C-MORE)

Principal Investigator:

David M. Karl (University of Hawaiʻi at Mānoa, SOEST)

Contact:

Mariona Segura-Noguera (University of Hawaiʻi at Mānoa, SOEST)

Technician:

Susan E. Curless (University of Hawaiʻi at Mānoa)

Data Manager:

Jasmine Nahorniak (Oregon State University, OSU-CEOAS)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Lynne M. Merchant (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-08-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Urea concentrations from multiple HOE-DYLAN cruises, R/V Kilo Moana KM1215, KM1217, KM1219, at Station ALOHA from July to September 2012 (C-MORE project)

Abstract:

In the summer of 2012, C-MORE conducted a "continuous" long-term field experiment at Station ALOHA to observe and interpret temporal variability in microbial processes, and the consequences for ecological dynamics and biogeochemical cycling. This dataset contains the N-urea measurements conducted during three cruises at station ALOHA, and were obtained at the nanomolar level using an autoanalyzer connected to a 2m LWCC, with methods developed by Mariona Segura-Noguera and Susan Curless.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-08-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.4055.1	2024-02-15

Description

Urea data from HOE-DYLAN casts.

In the summer of 2012, C-MORE conducted a "continuous" long-term field experiment at Station ALOHA to observe and interpret temporal variability in microbial processes, and the consequences for ecological dynamics and biogeochemical cycling. Special focus was given to time-space coupling because proper scale sampling of the marine environment is an imperative, but generally neglected aspect of marine microbiology.

Hawaii Ocean Experiment - Dynamics of Light and Nutrients (HOE-DYLAN)

Methods & Sampling

Seawater samples were collected from Niskin bottles with acid-washed syringes to avoid ammonium contamination with air and ship fumes. Samples were kept frozen (-20 ºC) until analysis. Urea was measured at the nanomolar level with the diacetyl monoxime automated method (Aminot and Kérouel, 1982), using a flow-injection system coupled to a 2m Liquid Waveguide Capillary Cell for reading the color. Low nutrient seawater exposed to daylight for two weeks was used for working standards preparation, blanks and carrier. The absorbance-concentration relationship was linear up to at least 800 nM Urea, with a method detection limit of 4.4 nM, and an error of 4.7% at 100nM.

BCO-DMO Processing

The date and time columns were combined to create an ISO datetime column named ISO_DateTime_UTC and of format YYYY-MM-DDThh:mmZ

Data Files

File
HOE_DYLAN cruise Urea observations filename: 4055_v1_hoe_dylan_cruises_urea.csv (Comma Separated Values (.csv), 15.08 KB) MD5:dd33136207334bbc289e3a6daea7751f File processed with laminar pipeline "4055_v1_HOE_DYLAN_Urea" at path 4055/1/data/4055_v1_hoe_dylan_cruises_urea.csv

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Biological Infrastructure	DBI-0424599

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
KM1215	HOE-DYLAN V	2012-07-08	R/V Kilo Moana	Samuel Wilson (Chief Scientist)	data info
KM1217	HOE-DYLAN VII	2012-08-05	R/V Kilo Moana	Sonya T. Dyhrman (Chief Scientist)	data info
KM1219	HOE-DYLAN IX	2012-08-22	R/V Kilo Moana	Samuel Wilson (Chief Scientist)	data info

Instruments

CTD Sea-Bird SBE 911plus

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird SBE 911plus

Acronym: CTD SBE 911plus

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/

Generic Description:

The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics

Fluorometer

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Liquid Waveguide Capillary Cells

Supplied Description:

These low level nutrient measurements were made with an LWCC (not the usual autoanalyzer). The methods were developed by Susan Curless and Mariona Segura-Noguera.

Instrument Type

Generic Name: Liquid Waveguide Capillary Cells

Acronym: LWCC

Generic Description:

Liquid Waveguide Capillary Cells (LWCC) are optical sample cells that combine an increased optical pathlength (2-500 cm) with small sample volumes. They can be connected via optical fibers to a spectrophotometer with fiber optic capabilities. Similar to optical fibers, light is confined within the (liquid) core of an LWCC by total internal reflection at the core/wall interface. Ultra-sensitive absorbance measurements can be performed in the ultraviolet (UV), visible (VIS) and near-infrared (NIR) to detect low sample concentrations in a laboratory or process control environment. According to Beer’s Law the absorbance signal is proportional to chemical concentration and light path length.

Niskin bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	Cruise ID	unitless	cruise_id
ISO_DateTime_UTC	Date and time (UTC) formatted to ISO 8601 standard Format: yyyy-MM-ddTHH:mmZ	unitless	ISO_DateTime_UTC
sta	station number	unitless	sta
cast	Cast number	unitless	cast
bot	Rosette bottle number	unitless	bot
lat	Station latitude; south is negative	decimal degrees	lat
lon	Station longitude; west is negative	decimal degrees	lon
press	Pressure from CTD	dbar	press
temp	Temperature from CTD	degrees Celcius	temp
sal	Salinity from CTD	PSS-78	sal
O2	Dissolved Oxygen from CTD	micromoles/kilogram	O2
fluor_re	Rescaled fluorescence from CTD estimate Chlorophyll	mircorgrams/liter	fluor_re
Urea	N-Urea	nanomoles/liter	Urea
Urea_sd	Standard deviation of Urea	unitless	Urea