Dataset: Tricho N Atlantic - OC469

Name: Trichodesmium species in the North Atlantic from R/V Oceanus OC469-01 in the NW Atlantic, Woods Hole to Barbados from October 2010 (Trichodesmium project)
Published: 2014-04-08
Keywords: oceans

Cite This Dataset

Spatial Extent: N:31.6867 E:-48.4535 S:11.5365 W:-67.30283

Project:

Quantification of Trichodesmium spp. vertical and horizontal abundance patterns and nitrogen fixation in the western North Atlantic (Trichodesmium)

Program:

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Dennis J. McGillicuddy (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Co-Principal Investigator:

Cabell S. Davis (Woods Hole Oceanographic Institution, WHOI)

Sonya T. Dyhrman (Woods Hole Oceanographic Institution, WHOI)

Dr John Waterbury (Woods Hole Oceanographic Institution, WHOI)

Data Manager:

Olga Kosnyrev (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-04-08

Version Date:

2014-04-08

Validated:

Yes

Current State:

Final no updates expected

Trichodesmium species in the North Atlantic from R/V Oceanus OC469-01 in the NW Atlantic, Woods Hole to Barbados from October 2010 (Trichodesmium project)

Expand/Collapse All

Description

At each station, CTD casts measured temperature, salinity and PAR. Water samples collected at depths of 700, 500, 300, 200, 100, 80, 60, 40, 20 m, and the surface were filtered and preserved for nutrient analysis. In the upper 80 m, water samples were gravity filtered and preserved for microscopic enumeration of both Trichodesmium colonies and free trichomes. For each nitrogen fixation sample, the number of puffs, number of rafts, and amount of carbon was measured. Individual carbon per colony values were estimated by regressing carbon content with number of puffs and number of rafts. Bowtie carbon content per colony was assumed the same as puff carbon per colony.

The sampling program included daily stations with associated nitrogen fixation experiments beginning at approximately 10:00 a.m. local time. Trichodesmium colonies for on-board incubation experiments and genetic assays were picked individually with pipettes from water collected at the surface (5-15 m) and at depth (20-70 m). Surface and deep samples were collected by pumping water through a 150 µm sieve on OC469 and by MOCNESS with 150 µm nets on OC471. Additional surface samples were taken by net tow (150 µm) on both cruises. After initial collection, the largest and most intact individual colonies were isolated using eyedroppers and transferred to filtered seawater for incubation experiments in order to assemble sufficient biomass to produce measurable rates. Nitrogen fixation was measured by acetylene reduction assay (Capone and Montoya, 2001).

Related References:

Capone, D. G. and J. P. Montoya, 2001: Nitrogen fixation and denitrification. Marine Microbiology, J. H. Paul, Ed., Academic Press, Methods in Microbiology, Vol. 30, 501-515, doi: http://dx.doi.org/10.1016/S0580-9517(01)30060-0, URL: http://www.sciencedirect.com/science/article/pii/S0580951701300600.

Related Dataset:

Tricho N Atlantic - OC471: http://www.bco-dmo.org/dataset/505567

Data Processing Description

See Nutrients Detection Limit (DL) and Quality Limit (QL): OC469 and OC471 (pdf)

See Experimental Treatment Codes (OC469) (pdf)

See OC469 CN Molar CN ID codes (pdf)

See Readme file (pdf)

See data corrections file (pdf)

Significance code descriptions:

1 - Values that were not statistically significant (e.g. slopes were not statistically greater than zero as determined using Prism software) - these cells were formatted bold, italic in the original data file.

2 - Values that were not statistically significant (e.g. slopes were not statistically greater than zero as determined using Prism software) - these cells were formatted bold, italic in the original data file. DEAD? (oc471, st15, 6 points)

3 - Monica Ruoco: not considered. They might be almost dead (oc469, st13)

nd - no notes

Data Files

File
tricho_oc469_8apr2014.csv (Comma Separated Values (.csv), 189.61 KB) MD5:ec68e1623fda7b730bda0ecb22fad591 Primary data file for dataset ID 472813

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0925284

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
OC469-01		2010-10-02	R/V Oceanus	Dennis J. McGillicuddy (Chief Scientist)	data info

Instruments

CTD - profiler

Supplied Name: CTD

Supplied Description:

SeaBird 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors

Instrument Type

Generic Name: CTD - profiler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.

This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934.

LI-COR Biospherical PAR Sensor

Supplied Name: LI-COR Biospherical PAR

Supplied Description:

Instrument Type

Generic Name: LI-COR Biospherical PAR Sensor

Acronym: LI-COR Biospherical PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0074/

Generic Description:

The LI-COR Biospherical PAR Sensor is used to measure Photosynthetically Available Radiation (PAR) in the water column. This instrument designation is used when specific make and model are not known.

Photosynthetically Available Radiation Sensor

Supplied Name: PAR sensor

Supplied Description:

Biospherical underwater PAR (1000m depth limit) with reference Surface PAR

Instrument Type

Generic Name: Photosynthetically Available Radiation Sensor

Acronym: PAR sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/122/

Generic Description:

A PAR sensor measures photosynthetically available (or active) radiation. The sensor measures photon flux density (photons per second per square meter) within the visible wavelength range (typically 400 to 700 nanometers). PAR gives an indication of the total energy available to plants for photosynthesis. This instrument name is used when specific type, make and model are not known.

Plankton Net

Supplied Description:

150 micron mesh on a 1-meter ring net

Instrument Type

Generic Name: Plankton Net

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/22/

Generic Description:

A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available.

Pressure Sensor

Supplied Description:

Digiquartz

Instrument Type

Generic Name: Pressure Sensor

Generic Description:

A pressure sensor is a device used to measure absolute, differential, or gauge pressures. It is used only when detailed instrument documentation is not available.

Pump

Supplied Name:

Supplied Description:

On OC-469-01, seawater from surface and deep samples were pumped through a 150 micron sieve.

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Sea-Bird SBE 43 Dissolved Oxygen Sensor

Supplied Name: SBE-43 DO

Supplied Description:

Instrument Type

Generic Name: Sea-Bird SBE 43 Dissolved Oxygen Sensor

Acronym: SBE-43 DO

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0036/

Generic Description:

The Sea-Bird SBE 43 dissolved oxygen sensor is a redesign of the Clark polarographic membrane type of dissolved oxygen sensors. more information from Sea-Bird Electronics

Seapoint Turbidity Meter

Supplied Name: Seapoint Turbidity

Supplied Description:

Instrument Type

Generic Name: Seapoint Turbidity Meter

Acronym: Seapoint Turbidity

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0104/

Generic Description:

The Seapoint Turbidity Meter detects light scattered by particles suspended in water, generating an output voltage proportional to turbidity or suspended solids.

Transmissometer

Supplied Description:

Wet Labs C*Star transmissometer (660nm wavelength)

Instrument Type

Generic Name: Transmissometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/124/

Generic Description:

A transmissometer measures the beam attenuation coefficient of the lightsource over the instrument's path-length. This instrument designation is used when specific manufacturer, make and model are not known.

Wet Labs ECO-AFL/FL Fluorometer

Supplied Name: ECO AFL/FL

Supplied Description:

Instrument Type

Generic Name: Wet Labs ECO-AFL/FL Fluorometer

Acronym: ECO AFL/FL

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0172/

Generic Description:

The Environmental Characterization Optics (ECO) series of single channel fluorometers delivers both high resolution and wide ranges across the entire line of parameters using 14 bit digital processing. The ECO series excels in biological monitoring and dye trace studies. The potted optics block results in long term stability of the instrument and the optional anti-biofouling technology delivers truly long term field measurements.
more information from Wet Labs

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
station	station number	unitless	station
cast	CTD cast number	unitless	cast
date	CTD date	yyyymmdd	date
time	CTD time	hhmm	time
year	year	unitless	year
month	month	unitless	month
day	day	unitless	day
yrday_gmt	GMT day and decimal time; as 326.5 for the 326th day of the year or November 22 at 1200 hours (noon)	unitless	yrday_gmt
inst_Tricho	Trichodesmium sampling instrument (pump or net)	unitless	instrument
cast2	pump cast number	unitless	cast
date_cast2	pump date	yyyymmdd	date
time_cast2	pump time	hhmm	time
lat_cast2	pump station latitude; north is positive	decimal degrees	lat
lon_cast2	pump station longitude; east is positive	decimal degrees	lon
lat	CTD latitude	decimal degrees	lat
lon	CTD longitude	decimal degrees	lon
depth_n	nominal depth	meters	depth_n
press	pressure	decibars	press
num_BTL	# of .BTL values used to compute average CTD pressure temperature salinity etc reported for that depth entry in the bottle file. [.BTL files are created by SeaBird CTD processing with average values for the time each bottle was tripped.]	unitless	no_bcodmo_term
NH4	ammonium concentration	microMolar	NH4
NO3_NO2	nitrate and nitrite concentration	microMolar	NO3_NO2
DIN	dissolved inorganic nitrogen concentration	microMolar	no_bcodmo_term
TDN	total dissolved nitrogen concentration	microMolar	TDN
DON	dissolved organic nitrogen concentration	microMolar	DON
TDP	total dissolved phosphorus concentration	microMolar	TDP
PO4	Phosphate concentration	microMolar	PO4
DOP	dissolved organic phosphorous concentration	microMolar	DOP
Si	silicate concentration	microMolar	Si
PO4_P_flag	PO4-P low level dissolved inorganic phosphate (LLDIP) assay marker: 1 = LLDIP was used (typically in upper ocean samples); 0 = the standard method was used (typically deeper samples where DIP is higher). See nutrient detection limit note (pdf) in Processing section.	unitless	no_bcodmo_term
O2_ml_L	dissolved oxygen concentration	milliliters/liter	O2_ml_L
sal	salinity from primary sensor	practical salinity units	sal
sal2	salinity from secondary sensor	practical salinity units	sal2
density	sigma-theta density from primary sensor	kilograms/meter^3	density
density2	sigma-theta density from secondary sensor	kilograms/meter^3	density
temp	temperature from primary sensor	degrees Celsius	temp
temp2	temperature from secondary sensor	degrees Celsius	temp2
cond	conductivity from primary sensor	Siemens/meter	cond
cond2	conductivity from secondary sensor	Siemens/meter	cond2
fluor	fluorescence	milligrams/m^3	fluor
trans	beam transmission	percent	trans
alt	altitude	meters	altitude
par	PAR/Irradiance	microEinsteins/cm^2/second	PAR
spar	SPAR/Surface Irradiance	microEinsteins/cm^2/second	SPAR
turbidity	turbidity	Nephelometric Turbidity Units (NTU)	turbidity
O2_v	oxygen voltage	volts	O2_v
AP_activity	Water column alkaline phosphatase activity	nanomoles Phosphate/hour/liter	no_bcodmo_term
chl_a	chlorophyll	micrograms/liter	chl_a
Trich_AP_mix	Trichodesmium AP Activity - Mixed	nanomoles Phosphorus/hour/colony	no_bcodmo_term
Trich_AP_puff	Trichodesmium AP Activity - Puffs	nanomoles Phosphorus/hour/colony	no_bcodmo_term
Trich_AP_raft	Trichodesmium AP Activity - Rafts	nanomoles Phosphorus/hour/colony	no_bcodmo_term
light_insitu	in situ light level	microEinsteins	no_bcodmo_term
light_incub	incubation light level	microEinsteins	no_bcodmo_term
temp_incub	incubation temperature	degrees Celsius	temp_inc
Nfix_colony_1	N fixation rate - colony 1	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_colony_1_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_colony_2	N fixation rate - colony 2	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_colony_2_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_colony_3	N fixation rate - colony 3	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_colony_3_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_colony_avg	average colony nitrogen fixation rate	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_colony_avg_sd	standard deviation of colony average nitrogen fixation rate	nanomoles Nitrogen/hour/colony	std_dev
num_rafts_1	number rafts - replicate 1	rafts	count
num_rafts_2	number rafts - replicate 2	rafts	count
num_rafts_3	number rafts - replicate 3	rafts	count
num_puffs_1	number puffs - replicate 1	puffs	count
num_puffs_2	number puffs - replicate 2	puffs	count
num_puffs_3	number puffs - replicate 3	puffs	count
Nfix_C_1	nitrogen fixation rate - replicate 1	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_C_1_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_C_2	nitrogen fixation rate - replicate 2	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_C_2_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_C_3	nitrogen fixation rate - replicate 3	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_C_3_sig	significance code: see codes in Processing section	unitless	no_bcodmo_term
Nfix_C_avg	average nitrogen fixation rate	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_C_sd	standard deviation of nitrogen fixation rate	micromoles Nitrogen/hour/mole Carbon	std_dev
expt_code	experimental treatment code. See notes (pdf) in Processing section	unitless	no_bcodmo_term
Nfix_exp_colony_1	experimental nitrogen fixation rate - colony 1	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_exp_colony_2	experimental nitrogen fixation rate - colony 2	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_exp_colony_3	experimental nitrogen fixation rate - colony 3	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_exp_colony_avg	average colony experimental nitrogen fixation rate	nanomoles Nitrogen/hour/colony	no_bcodmo_term
Nfix_exp_colony_sd	standard deviation of colony experimental nitrogen fixation rate	nanomoles Nitrogen/hour/colony	std_dev
Nfix_exp_C_1	experimental nitrogen fixation rate - replicate 1	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_exp_C_2	experimental nitrogen fixation rate - replicate 2	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_exp_C_2_sig	significance note: see codes in Processing section	unitless	no_bcodmo_term
Nfix_exp_C_3	experimental nitrogen fixation rate - replicate 3	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_exp_C_avg	average experimental nitrogen fixation rate	micromoles Nitrogen/hour/mole Carbon	no_bcodmo_term
Nfix_exp_C_sd	standard deviation of experimental nitrogen fixation rate	micromoles Nitrogen/hour/mole Carbon	std_dev
num_rafts_exp_1	number of rafts in experimental treatment - replicate 1	rafts	count
num_rafts_exp_2	number of rafts in experimental treatment - replicate 2	rafts	count
num_rafts_exp_3	number of rafts in experimental treatment - replicate 3	rafts	count
num_puffs_exp_1	number of puffs in experimental treatment - replicate 1	puffs	count
num_puffs_exp_2	number of puffs in experimental treatment - replicate 2	puffs	count
num_puffs_exp_3	number of puffs in experimental treatment - replicate 3	puffs	count
CNMolarC_N_id	molar carbon to nitrogen ratio identification codes???	unitless	no_bcodmo_term
C_exp_colony	carbon content per colony in experimental treatment	micromoles Carbon	no_bcodmo_term
N_exp_colony	nitrogen content per colony in experimental treatment	micromoles Nitrogen	no_bcodmo_term
C_to_N_exp_colony	carbon to nitrogen ratio per colony in experimental treatment	unitless	C_to_N
num_colony_puff	number of puff colony forms	colonies	count
num_colony_raft	number of raft colony forms	colonies	count
num_colong_bow	number of bowtie colony forms	colonies	count
num_colony_totl	number of total colony forms	colonies	count
filament_free	number of free filaments	filaments	count
vol_filt_colony_filamt	volume filtered for colonies and filaments	liters	vol_filt
ISODateTime_UTC	Date/Time (UTC) ISO formatted. E.g., 2009-08-30T14:05:00[.xx]Z (UTC time)	YYYY-MM-DDTHH:MM:SS[.xx]Z	ISO_DateTime_UTC

Status message

Database

Contribute Data

Dataset: Tricho N Atlantic - OC469