Dataset: diatom_abundance_Ant1

Name: Diatom abundances from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project)
Published: 2014-02-06
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-75 E:177.36 S:-75 W:177.36

Project:

Controls of Ross Sea Algal Community Structure (CORSACS)

Rising climatic temperatures impact on antarctic microzooplankton growth and grazing (Antarctic microzooplankton )

Program:

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Dr Julie Rose (National Oceanic and Atmospheric Administration , NOAA-Milford)

Co-Principal Investigator:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-02-06

Version Date:

2014-02-06

Validated:

Yes

Current State:

Final no updates expected

Diatom abundances from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project)

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Description

Experimental Design

Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.

References

Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.

Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.

Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.

Methods & Sampling

Total diatom abundance and community composition was determined for samples preserved with 1% glutaraldehyde (final concentration) and stored in the dark at 4 deg C until analysis. Twenty-five mL samples were settled in Utermöhl chambers for at least 18 h and enumerated using light microscopy at 400x magnification with a Zeiss Axiovert S100 inverted microscope (Utermohl, 1958). Diatoms were identified to the genus level.

References

Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.

Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.

Data Processing Description

BCO-DMO Processing Notes:

- File was sorted by treatment
- Added lat,lon values of original water sampling location to file
- Added BCO-DMO header line
- File was transposed to serve data by taxon and abundance in columns

Data Files

File
diatom_abund.csv (Comma Separated Values (.csv), 19.39 KB) MD5:943a312cce02216e23175056df3b6e69 Primary data file for dataset ID 490884

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Antarctic Sciences	PLR-0528715

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
NBP0601	CORSACS I	2005-12-17	RVIB Nathaniel B. Palmer	Giacomo DiTullio (Chief Scientist)	data info

Instruments

Inverted Microscope

Supplied Description:

Samples were enumerated using light microscopy at 400x magnification with a Zeiss Axiovert S100 inverted microscope (Utermohl, 1958).

Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.

Instrument Type

Generic Name: Inverted Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).

Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.

The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
lat	Latitude component of geographic position where water was sampled.	decimal degrees	lat
lon	Longitude component of geographic position where water was sampled.	decimal degrees	lon
treatment	Experimental conditions varied during the experiment. Four treatments were used: Low temperature, low iron (LTLF); low temperature, high iron (LTHF); high temperature, low iron (HTLF); high temperature, high iron(HTHF)	dimensionless	treatment
day	Sampling day during experiment. The experiment was conducted during January, 2006.	dimensionless	time_inc
bottle	Experimental bottle number.	dimensionless	no_bcodmo_term
abundance	Number of cells of each taxa per ml.	cells/ml	abundance
taxon	Taxanomic name of each organism counted.	dimensionless	taxon

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Dataset: diatom_abundance_Ant1