Dataset: Symbiodinium culture collection

Name: Cnidarian symbiont culture collection (BURRCC) collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)
Published: 2015-01-15
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:43 E:-78.7914 S:43 W:-78.7914

Project:

Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)

Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2015-01-15

Version Date:

2015-01-15

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Cnidarian symbiont culture collection (BURRCC) collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)

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Description

Summary of Symbiodinium cultures in the BURR Culture Collection with symbiont phylotype based on variation in the chloroplast 23S-rDNA. Collections made world-wide; cultures are maintained at the University at Buffalo. Cultures are available to researchers for a nominal charge. Contact coffroth@buffalo.edu for further information.

This dataset provides the Symbiodinium clade (Rowan and Powers, 1991, Science 251: 1348-1351) and variation in domain V of the chloroplast 23S rDNA (Santos et al 2003, Mar.Biotech. 5:130-140).

Methods & Sampling

Symbionts isolated from cnidarian fragments and reared in F/2 media under 40W cool-white lights at 26oC on a 14:10h light:dark cycle. Symbiont identification followed that of Santos et al. 2003, Mar.Biotech. 5:130-140.

Related Reference:

- Santos SR, Gutiérrez-Rodríguez C, Coffroth MA (2003) Phylogenetic identification of symbiotic dinoflagellates via length heteroplasmy in domain V of chloroplast large subunit (cp23S)-rDNA sequences. Mar Biotech 5: 130-140 for technique to characterize symbionts based on of chloroplast large subunit (cp23S)-rDNA.
- Rowan, R. and D. Powers (1991). A molecular genetic classification of zooxanthellae and the evolution of animal-algal symbioses. Science 251: 1348-1351 for technique to characterize symbionts at the clade level.
- BURR webpage (http://www.nsm.buffalo.edu/Bio/burr/) for details of symbiont isolation and culturing.

Data Processing Description

BCO-DMO Processing:

- original file: Coffroth_BURR Culture Collection database_Dec 2014.xlsx
- added conventional header with dataset name, PI name, version date, reference information
- changed parameter names to be BCO-DMO compatible
- replaced spaces with underscores

Data Files

File
burr.csv (Comma Separated Values (.csv), 16.07 KB) MD5:8f4bac396fcdd48990c9df3957f10817 Primary data file for dataset ID 546049

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0926822
NSF Division of Ocean Sciences	OCE-9907319
NSF Division of Ocean Sciences	OCE-0424996

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Coffroth_2010		2010-01-01	SUNY-Buffalo	Mary Alice Coffroth (Principal Investigator)	data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory identification; for mapping puposes	unitless	laboratory
lat	latitude of lab; north is positive	decimal degrees	lat
lon	londitude of lab; east is positive	decimal degrees	lon
culture_id	Symbiodinium culture identification	unitless	no_bcodmo_term
clade	Symbiodinium clade	unitless	no_bcodmo_term
cp_23S_rDNA_genotype	variation in domain V of the chloroplast 23S rDNA	unitless	no_bcodmo_term

Status message

Database

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Dataset: Symbiodinium culture collection