Dataset: Grazing experiment 4: daily cell counts

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml.  Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients.  Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts.  Cells were counted live on a Z2 Coulter Particle Counter.  The dilution volume was then calculated to achieve a cell density of approximately 25,000 cells per ml which was the density determined in preliminary experiments to be adequate to maintain pCO2 near target concentrations.