Dataset: Grazing experiment 5: daily cell counts

Name: Grazing experiments 5: Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. (E Hux Response to pCO2 project)
Published: 2016-12-09
Keywords: biota, oceans

Cite This Dataset

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-12-09

Restricted:

Release Date:

2017-12-31

Validated:

Yes

Current State:

Final no updates expected

Grazing experiments 5: Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. (E Hux Response to pCO2 project)

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml. Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients. Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts. Cells were counted live on a Z2 Coulter Particle Counter. The dilution volume was then calculated to achieve a cell density of approximately 25,000 cells per ml which was the density determined in preliminary experiments to be adequate to maintain pCO2 near target concentrations.

Data Processing Description

These data are unprocessed cell counts.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
expt5_cell_count_daily.csv (Comma Separated Values (.csv), 2.30 KB) MD5:578cbc077c724d31b58c4e790192ef53 Primary data file for dataset ID 669572

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0961229

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

Coulter Counter

Supplied Name: Z2 Coulter Particle Counter

Supplied Description:

Used to count cells

Instrument Type

Generic Name: Coulter Counter

Generic Description:

An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.

from https://en.wikipedia.org/wiki/Coulter_counter

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment_replicate	sample identifier: individual grazer analyzed	unitless	sample
target_density_day_1	Target count for day 1 morning cell count	cells/milliliter	count
count_day_2	Count for day 2 morning cell count	cells/milliliter	count
day_2_post_dilution	Count for day 2 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_3	Count for day 3 morning cell count	cells/milliliter	count
day_3_post_dilution	Count for day 3 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_4	Count for day 4 morning cell count	cells/milliliter	count
day_4_post_dilution	Count for day 4 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_5	Count for day 5 morning cell count	cells/milliliter	count
day_5_post_dilution	Count for day 5 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_6	Count for day 6 morning cell count	cells/milliliter	count
day_6_post_dilution	Count for day 6 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_7	Count for day 7 morning cell count	cells/milliliter	count
day_7_post_dilution	Count for day 7 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_8	Count for day 8 morning cell count	cells/milliliter	count
day_8_post_dilution	Count for day 8 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_9	Count for day 9 morning cell count	cells/milliliter	count
day_9_post_dilution	Count for day 9 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_10	Count for day 10 morning cell count	cells/milliliter	count
day_10_post_dilution	Count for day 10 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_11	Count for day 11 morning cell count	cells/milliliter	count
day_11_post_dilution	Count for day 11 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_12	Count for day 12 morning cell count	cells/milliliter	count
day_12_post_dilution	Count for day 12 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_13	Count for day 13 morning cell count	cells/milliliter	count
day_13_post_dilution	Count for day 13 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_14	Count for day 14 morning cell count	cells/milliliter	count
day_14_post_dilution	Count for day 14 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_15	Count for day 15 morning cell count	cells/milliliter	count
day_15_post_dilution	Count for day 15 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_16	Count for day 16 morning cell count	cells/milliliter	count
day_16_post_dilution	Count for day 16 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
count_day_17	Count for day 17 morning cell count	cells/milliliter	count

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Dataset: Grazing experiment 5: daily cell counts