Dataset: McIlroy_2016: Effective quantum yield for Orbicella faveolata recruits

Collection of Orbicella faveolata larvae was made at Alligator Reef (24°48.7710 N, 80°40.1670 W) and Looe Key (24°32.6930 N, 81°24.5620 W) in 2011. Initial rearing was done at Keys Marine Laboratory (KML), Long Key, FL. Settled corals were then maintained at University of Miami Experimental Hatchery (Virginia Key, Miami, FL). Symbiodinium cultures originated from highly concentrated, isoclonal reference cultures maintained at the University at Buffalo – SUNY (BURR Culture Collection: http://www.nsm.buffalo.edu/Bio/burr/). The cultures used in the experiments were maintained at the Keys Marine Lab (Florida) in f/2 medium (Guillard 1975), at ~27°C, under a 14:10 h light:dark regime.

Measures of photophysiology for recruits harboring either S. microadriaticum or S. minutum were determined using an imaging-PAM (WALZ Mess- und Regeltechnik, Germany). All fluorescence parameters were described and abbreviated according to Cosgrove and Borowitzka (2010) and Enriquez and Borowitzka (2010). For the first 6 months following settlement (age in months 1-6) analyses were run monthly on one designated tile per tank (n = 4 tiles). We measured the effective quantum yield (delta Fv/Fm0', the efficiency of photochemistry in the light-adapted state) for six randomly selected recruits from each tile repeated monthly for 6 months. Data were analyzed using a two-way ANOVA, with symbiont treatment and time as factors and tile nested within treatment.