Dataset: Series 1A: light and temperature

Name: Series 1A: Two-stressor effects on Tp-1335: Experiments to investigate the combined effect of light and temperature changes on the growth rate of Thalassiosira pseudonana CCMP
Published: 2017-09-11
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.714528.1

Temporal Extent: 2016-04-15

Project:

Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Principal Investigator:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2017-09-11

Restricted:

Release Date:

2020-08-31

Validated:

Yes

Current State:

Final no updates expected

Series 1A: Two-stressor effects on Tp-1335: Experiments to investigate the combined effect of light and temperature changes on the growth rate of Thalassiosira pseudonana CCMP

Abstract:

Series 1A: Two-stressor effects on Tp-1335: Experiments were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335. This is the first series, consisting of 5 experiments

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2017-09-11	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.714528.1	2020-08-31

Description

Experiments were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335. T. pseudonana was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975). Each of five experiments per series was conducted at a different temperature (13.4; 18.5; 24.4; 26.5; 22.5 C). At each temperature, cultures were kept on a 12-hour light 12-hour dark cycle under eight light intensities ranging from 35 umol/m^2/S to 140 umol/m^2/s. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope.

Date Expt# Temperature (°C)
04/15/2016 1 13.4
05/30/2016 2 18.5
07/31/2016 3 24.4
08/20/2016 4 26.5
08/22/2016 5 22.5

Methods & Sampling

Problem Report:
Follow-up experiments indicated that growth rates during these experiments were not maximum for the respective temperature and irradiance conditions. The most likely explanation is growth limitation due to some trace element not present in artificial seawater (ASW). Whereas this species originally grew well in ASW, growth rates dropped under all environmental conditions after several weeks. Experiments were repeated in Series 1B, and cultures grown in ASW with 5% UV-sterilized seawater added.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- replaced blank cells with 'nd' (no data)

Data Files

File
Thal_pseudonana_growth_series1.csv (Comma Separated Values (.csv), 13.63 KB) MD5:19bb696950c6c68533c8dc2fc01a5a8f Primary data file for dataset ID 714528

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1538602

Deployments

None available yet

Instruments

Cell Cultivator

Supplied Name: Multicultivator MC-1000 OD (Qubit Systems)

Supplied Description:

Used for incubations and optical density measurements.

Instrument Type

Generic Name: Cell Cultivator

Generic Description:

An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.

Fluorometer

Supplied Name: Z985 Cuvette Aquapen (Qubit Systems)

Supplied Description:

Used to measure instantaneous chlorophyll fluorescence (F0) and quantum Yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity).
A 20-minute dark adaptation time was used.
AquaPen settings: f = 30, F=71, A = 50.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
expt	experiment identifier	unitless	exp_id
date_local	date of experiment (local time)	unitless	date_local
temperature	temperature of culture	degrees Celsius	temp
Light	irradiance: L1=35 µmol m-2 s-1; L2= 50 µmol m-2 s-1; L3=65 µmol m-2 s-1; L4=80 µmol m-2 s-1; L5=95 µmol m-2 s-1; L6=110 µmol m-2 s-1; L7=125 µmol m-2 s-1; L8=140 µmol m-2 s-1	micromole quanta /meter^2/second	irradiance
Day	day of incubation	days	time_inc
OD680_avg	optical density at 680 nm; averaged from 7 measurements taken automatically in 10 minute intervals.	unitless	optical_density
OD720_avg	optical density at 720 nm; averaged from 7 measurements taken automatically in 10 minute intervals.	unitless	optical_density
F0	instantaneous chlorophyll fluorescence (F_0)	relative units	fluor
count_cells_ml	cell concentrations	cells per milliliter	cell_concentration
QY	QY=F_v/F_m where F_v is the maximal variable fluorescence and F_m is the maximal fluorescence intensity	unitless	Fv_Fm

Status message

Database

Contribute Data

Dataset: Series 1A: light and temperature