Dataset: Plocamium: pH and temperature time-series

Name: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)
Published: 2018-03-19
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.731134.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2014-07-24 - 2015-02-23

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSUN)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-03-19

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)

Abstract:

Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-03-19	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.731134.1	2019-06-03

Description

This dataset includes measurements of temperature and pH from culture pots of Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015.

Related Datasets:
Plocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)

Plocamium culture carbonate chemistry: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)

Plocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)

Plocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)

Plocamium growth and biomass: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)

Plocamium pigments: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)

Plocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)

Rapid Light Curves_PAM: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions

Methods & Sampling

Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.

In situ probes in each culture pot monitored pH and temperature every 10 minutes by sampling and logging values to an Excel spreadsheet to track the dynamics of both variables.

Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.

See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- formatted trial#5 date and time to be consistent with other trials
- added columns date, time, ISO_DateTime_Local

Data Files

File
pH_temp_timeseries.csv (Comma Separated Values (.csv), 2.49 MB) MD5:e87caf813e2e0b3b8423fabad7c97ef2 Primary data file for dataset ID 731134

Supplemental Files

File

Table of culture conditions for each of the 8 trials
filename: Dudgeon_seaweed_trial_meta_2018-01.pdf

(Portable Document Format (.pdf), 429.04 KB)
MD5:5a4a9cbd3cb5092a419b4883c96979b8

Notes: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses. In each culture pot, pCO2 was set by the supply rate of CO2 in the corresponding mass-flow controlled gas mixing system to be within a target range of either near ambient, moderately elevated or highly elevated in each trial. The near-ambient range was narrower than the other target ranges (set points typically ~380 - 390 micro-atm) as it served as the control range in each trial. However, actual pCO2 in solution in each culture pot varied slightly from constant target values on a diurnal cycle associated with the metabolic activities of the algae contained within each pot. Unique average values of pCO2 in each culture pot based on different set values for each mass-flow controlled mixer within the qualitative ranges of ambient, moderate and highly increased pCO2 levels that were replicated in each trial of the experiment enabled a more powerful regression-type experimental design. With a regression type approach we could estimate the functional relationship between response variables and pCO2, which was not possible with a simple categorical treatment design.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

Aquarium chiller

Supplied Name: Aqua Logic Cyclone Chiller

Supplied Description:

Used to control the temperature of the growth chambers. A Neptune Systems Apex AquaController was used to measure the temperature and pH.

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date	local date	unitless	date_local
time	local time	unitless	time_local
ISO_DateTime_Local	Date/Time (local) ISO formatted based on ISO 8601:2004(E) with format YYYY-mm-ddTHH:MM:SS[.xx]	unitless	ISO_DateTime_Local
pH_S1P1	pH-Total scale in gas mixing system #1; Pot #1	unitless	pH
T_S1P1	Temperature in situ in gas mixing system #1; Pot #1	degrees Celsius	temp
pH_S1P2	pH-Total scale in gas mixing system #1; Pot #2	unitless	pH
T_S1P2	Temperature in situ in gas mixing system #1 ; Pot #2	degrees Celsius	temp
pH_S2P4	pH-Total scale in gas mixing system #2; Pot #3	unitless	pH
T_S2P4	Temperature in situ in gas mixing system #2; Pot #3	degrees Celsius	temp
pH_S3P7	pH-Total scale in gas mixing system #3; Pot #4	unitless	pH
T_S3P7	Temperature in situ in gas mixing system #3; Pot #4	degrees Celsius	temp
pH_S2P6	pH-Total scale in gas mixing system #2; Pot #5	unitless	pH
T_S2P6	Temperature in situ in gas mixing system #2; Pot #5	degrees Celsius	temp
pH_S3P8	pH-Total scale in gas mixing system #3; Pot #6	unitless	pH
T_S3P8	Temperature in situ in gas mixing system #3; Pot #6	degrees Celsius	temp
pH_S1P3	pH-Total scale in gas mixing system #1; Pot #7	unitless	pH
T_S1P3	Temperature in situ in gas mixing system #1; Pot #7	degrees Celsius	temp
pH_S2P5	pH-Total scale in gas mixing system #2; Pot #8	unitless	pH
T_S2P5	Temperature in situ in gas mixing system #2; Pot #8	degrees Celsius	temp
pH_S3P9	pH-Total scale in gas mixing system #3; Pot #9	unitless	pH
T_S3P9	Temperature in situ in gas mixing system #3; Pot #9	degrees Celsius	temp
Trial	Trial number	unitless	replicate