Dataset: A. pisonii: dissections

Name: Physiological parameters derived from dissection of mangrove crabs, A. pisonii, from three habitats along Florida's east coast in 2015 and 2016
Published: 2018-07-16
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.741114.1

Spatial Extent: N:30.1325 E:-80.28611 S:27.43 W:-81.38556

Temporal Extent: 2015-06-17 - 2016-09-04

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

Co-Principal Investigator:

Zachary J. Cannizzo (University of South Carolina)

Contact:

Zachary J. Cannizzo

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-07-16

Restricted:

Release Date:

2020-07-16

Validated:

Yes

Current State:

Final no updates expected

Physiological parameters derived from dissection of mangrove crabs, A. pisonii, from three habitats along Florida's east coast in 2015 and 2016

Abstract:

This dataset includes multiple physiological parameters derived from dissection of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-07-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.741114.1	2020-07-16

Description

This dataset includes multiple physiological parameters derived from dissection of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove.

Methods & Sampling

On each of 9 randomly selected days in each habitat, 15 individual adult A. pisonii were collected by hand and immediately placed on dry ice. In the mangrove and saltmarsh, we collected these crabs in three groups of five at three distinct tidal periods: just after losing access to the sediment on the flood tide, at slack high tide, and just before regaining access to the sediment on the ebb tide. Crabs were kept frozen until dissection. Prior to dissection, we determined the sex and carapace width of each crab. Upon dissection we ascertained gut-width as the width of the cardiac stomach.

We ascertained the gut fullness of each crab to obtain a snap-shot of the quantity of food consumed during each tidal period by removing the gut contents and drying them at 60-70oC to constant weight. We standardized gut fullness by dividing the mass of the gut contents by the volume of the gut ( ) where a is a correction factor of 0.92.

We also separated the hepatopancreas, reproductive tissue (gonads and eggs), feces still in the intestines, the gut, and the somatic tissue of each crab and dried them to a constant weight at 60-70oC.

The dry mass of each parameter was determined by subtracting the mass of the weigh boat used for that parameter from the dry mass of that parameter on the weigh-boat.

Hepatosomatic index was calculated as the ratio of the dry masses of the hepatopancreas and the somatic tissue.
Gonadosomatic index was calculated as the ratio of the dry masses of the reproductive tissues and the somatic tissue.
Gut volume was calculated using the equation ( ) where a is a correction factor of 0.92.

Locations: Florida East Coast.
Round Island Park: 27o33'33"N 80o19'53"W
Pepper Park: 27o29'42'N 80o18'12"W
North Causeway Park: 27o28'28"N 80o19'12"W
Oslo Road: 27o35'14"N 80o21'55"W
Anastasia State Park: 29o52'40"N 81o16'32"W
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W
Vilano Inlet: 29o55'16"N 81o17'57"W
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W
Boating Club Road: 29o56'34"N 81o18'31"W

Data Processing Description

BCO-DMO Processing
- added Year and ISO_Date columns; removed Date (d-Mon) column from display
- reduced decimal precision of GV, GW_CW from 9 to 3; HIS, GSI and GC_GV from 9 to 4

Data Files

File
Apisonii_dissection.csv (Comma Separated Values (.csv), 83.62 KB) MD5:706cb7f7a17b8d128243d84daf56967a Primary data file for dataset ID 741114

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1129166

Deployments

None available yet

Instruments

scale

Supplied Name:

Supplied Description:

Used to measure wet and dry weights of eggs

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
ID	Individual ID number given to each crab	unitless	individual
Habitat	The habitat where the crab was collected and observed	unitless	site_descrip
Site	Site of observation/collection. RI= Round Island Park; PP=Pepper Park; NC=North Causeway Park; Oslo=Oslo Road; BP=Bear Point; ANA=Anastasia State Park; GTM= Guana-Tolomato-Matanzas NERR; PV= Palm Valley/Nocatee Canoe Launch; YC=St. Augustine Yacht Club	unitless	site
Year	Year of collection	unitless	year
ISO_Date	Date in ISO format (yyyy-mm-dd)	unitless	date
Tide	Tidal period of collection. 1=As crabs lost access to sediment on the flood-tide; 2=Slack-high tide; 3=Just before crabs regained access to the sediment on the ebb-tide	unitless	no_bcodmo_term
Sex	Sex of crab	unitless	sex
Gravid	Reproductive state of female crabs. 0=non-ovigerous; 1-ovigerous (egg carrying)	unitless	no_bcodmo_term
CW	Size of crab; measured as carapace-width	mm	width
GW	Gut width measured as the width of cardiac stomach.	mm	width
GV	Gut volume measured as the volume of the cardiac stomach.	mm3	volume
GW_CW	gut-width:carapace-width ratio	unitless	no_bcodmo_term
S_WB	Mass of weight boat for somatic tissue.	grams	weight
S_Dry	Dry mass of somatic tissue on weigh boat.	grams	weight
S	Dry mass of somatic tissue.	grams	weight
F_WB	Mass of weigh-boat for feces.	grams	weight
F_Dry	Dry mass of feces on weigh-boat.	grams	weight
F	Dry mass of feces.	grams	weight
G_WB	Mass of weigh-boat for reproductive tissue.	grams	weight
G_Dry	Dry mass of reproductive tissue on weigh-boat.	grams	weight
G	Dry mass of reproductive tissue	grams	weight
H_WB	Mass of weigh-boat for hepatopancreas.	grams	weight
H_Dry	Dry mass of hepatopancreas on weigh-boat.	grams	weight
H	Dry mass of hepatopancreas.	grams	weight
GT_WB	Mass of weigh-boat for gut.	grams	weight
GT_Dry	Dry mass of gut on weigh-boat.	grams	weight
GT	Dry mass of gut.	grams	weight
GC_WB	Mass of weigh-boat for gut-content.	grams	weight
GC_Dry	Dry mass of gut-content on weigh-boat.	grams	weight
GC	Dry mass of gut-content.	grams	weight
HIS	Hepato-somatic index of crab	unitless	no_bcodmo_term
GSI	Gonado-somatic index of crab	unitless	no_bcodmo_term
GC_GV	Ratio of the mass of the gut content and the volume of the gut	unitless	no_bcodmo_term