Dataset: A. pisonii: egg lipid and glycogen

Name: Lipid and glycogen content of A. pisonii eggs from three habitats along the Florida eastern coast, 2016
Published: 2018-07-16
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.741192.1

Spatial Extent: N:30.1325 E:-80.28611 S:27.43 W:-81.38556

Temporal Extent: 2016-06-14 - 2016-10-13

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

Co-Principal Investigator:

Zachary J. Cannizzo (University of South Carolina)

Contact:

Zachary J. Cannizzo

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-07-16

Restricted:

Release Date:

2020-07-16

Validated:

Yes

Current State:

Final no updates expected

Lipid and glycogen content of A. pisonii eggs from three habitats along the Florida eastern coast, 2016

Abstract:

This dataset includes calculations of the lipid and glycogen content of eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-07-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.741192.1	2020-07-16

Description

Methods & Sampling

We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80^oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80^oC until analysis.

We used a modified Folch Extraction to extract the lipids from a subset (~20-40mg) of each clutch.

We used a subset (~10mg) of each clutch to compare the glycogen content of eggs between habitats. We did so using a Sigma-Aldrich Glycogen Assay Kit MAK016 and determined the glycogen concentration as a percentage of egg mass through colorimetry per the manufacturer instructions.

Mass of extracted lipids was calculated by subtracting the mass of the vial in which lipids was extracted from the combined mass of the lipids and the vial.

Glycogen concentration was calculated using colorimetry per the manufacturer instructions.

Locations, Florida East Coast:
Round Island Park: 27o33'33"N 80o19'53"W
Pepper Park: 27o29'42'N 80o18'12"W
North Causeway Park: 27o28'28"N 80o19'12"W
Oslo Road: 27o35'14"N 80o21'55"W
Anastasia State Park: 29o52'40"N 81o16'32"W
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W
Vilano Inlet: 29o55'16"N 81o17'57"W
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W
Boating Club Road: 29o56'34"N 81o18'31"W

Data Processing Description

BCO-DMO Processing:
- added ISO_Date columns; removed Month (number) and Date (d-Mon) column from display
- reduced decimal precision of: GW_CW from 9 to 3 places; Lipid_wt, Glycogen_Conc, Glycogen_pct (9 to 5)
- changed Glycogen_perc to Glycogen_pct to match Lipid_pct

Data Files

File
Apisonii_egg_lipid-glycogen.csv (Comma Separated Values (.csv), 16.20 KB) MD5:4e8ad2f9da1c13be563646694edfa1dd Primary data file for dataset ID 741192

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1129166

Deployments

None available yet

Instruments

bomb calorimeter

Supplied Name: Parr semimicro bomb calorimeter

Supplied Description:

Used to determine the energy content of the eggs.

Instrument Type

Generic Name: bomb calorimeter

Generic Description:

A device for determining heats of combustion by igniting a sample in a high pressure of oxygen in a sealed vessel and measuring the resulting rise in temperature.

scale

Supplied Name:

Supplied Description:

Used to measure wet and dry weights of eggs

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Habitat	The habitat where the crab was collected and observed.	unitless	site_descrip
Site	Site of observation/collection. RI= Round Island Park; PP=Pepper Park; NC=North Causeway Park; Oslo=Oslo Road; ANA=Anastasia State Park; GTM= Guana-Tolomato-Matanzas NERR; VM=Vilano Inlet; PV= Palm Valley/Nocatee Canoe Launch; YC=St. Augustine Yacht Club; BC=Boating Club Road	unitless	site
ISO_Date	Date in ISO format (yyyy-mm-dd)	unitless	date
ID	individual ID number given to each crab	unitless	individual
CW	Size of crab; measured as carapace-width	millimeters (mm)	width
GW	Gut width measured as the width of cardiac stomach.	millimeters (mm)	width
GW_CW	gut-width:carapace-width ratio	unitless	no_bcodmo_term
Egg_W	Dry mass of eggs from which lipid were extracted.	grams	weight
F_Vial	Mass of final vial in-which extracted lipids was weighed.	grams	weight
Vial_Lipid	Mass of extracted lipids in vial.	grams	lipids_biota
Lipid	Mass of extracted lipids.	grams	lipids_biota
Lipid_wt	Proportion of lipid mass to mass of eggs from which the lipids were extracted	unitless	lipids_biota
Lipid_pct	Concentration of egg lipids in mass-percent of eggs from which lipids were extracted	unitless	lipids_biota
Glycogen_W	Mass of eggs from which glycogen was extracted.	milligrams	weight
Glycogen_Conc	Proportion of glycogen mass to mass of eggs from which the glycogen was extracted	unitless	no_bcodmo_term
Glycogen_pct	Concentration of egg glycogen in mass-percent of eggs from which glycogen was extracted	unitless	no_bcodmo_term