Dataset: Series 1B: light and temperature on growth follow-up expts

Name: Series 1B: Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments
Published: 2018-09-04
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.745492.1

Temporal Extent: 2017-05-08 - 2017-07-07

Project:

Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Principal Investigator:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

Student:

Julia Sweet (University of California-Santa Barbara, UCSB-MSI)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-09-04

Restricted:

Release Date:

2020-08-31

Validated:

Yes

Current State:

Final no updates expected

Series 1B: Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments

Abstract:

This dataset reports supplemental experiments that were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335. Experiments were conducted at three temperatures and two light levels. Optical density measurements, dark-adapted Instantaneous Chlorophyll Fluorescence, and the quantum yield and cell concentrations were determined daily. Particulate organic carbon and nitrogen as well as Chl a were measured when volume allowed.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-09-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.745492.1	2020-08-31

Description

Methods & Sampling

T. pseudonana was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975) with 50mL autoclaved natural filtered seawater per liter added. Experiments were conducted at 13.5, 22, and 26˚C. Cultures were kept on a 12-hour light 12-hour dark cycle under light intensities ranging from 35 µmol m-2 s-1 to 350 µmol m-2 s-1. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope. Cell concentrations on day 0 were calculated based on the inoculum and the dilution. Particulate organic carbon and nitrogen as well as Chl a were measured when volume allowed. Combusted 25mm GFF were prepared for particulate organic carbon and particulate organic nitrogen (POC, PON) and measured in a CEC44OHA elemental analyzer (Control Equipment). Chl a was collected on 47mm 0.45 HAWP filters, then extracted in 90% acetone for 24hrs and measured in a fluorometer (Turner 700).

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- replaced blank cells with 'nd' (no data)
- combined data for all four experiments into one table (dataset)
- added columns for experiment identifier, temperature, light level and date of experiment
- reformatted integers to remove embedded commas

Data Files

File
Tp-growth-S1B.csv (Comma Separated Values (.csv), 13.31 KB) MD5:bd69930d2f14953c6dc2e22c142f6b88 Primary data file for dataset ID 745492

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1538602

Deployments

None available yet

Instruments

Cell Cultivator

Supplied Name: Multicultivator MC-1000 OD (Qubit Systems)

Supplied Description:

Used for incubations and optical density measurements.

Instrument Type

Generic Name: Cell Cultivator

Generic Description:

An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.

Fluorometer

Supplied Name: Z985 Cuvette Aquapen (Qubit Systems)

Supplied Description:

Used to measure instantaneous chlorophyll fluorescence (F₀) and quantum Yield (QY=F_v/F_m, where F_v is the maximal variable fluorescence and F_m is the maximal fluorescence intensity).
A 20-minute dark adaptation time was used.
AquaPen settings: f = 30, F=71, A = 50.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
expt	experiment identifier	unitless	exp_id
temp	experimental temperature	degrees Celsius	temp
light_level	experimental light level	unitless	no_bcodmo_term
date_expt	date of experiment formatted as yyyy-mm-dd	unitless	date
light	irradiance	micromoles/square meter/second (umol m-2 s-1)	irradiance
day	sampling day where day 0 equals inoculation	day	day
avg_OD680	optical density at 680 nm; averaged from 7 measurements taken automatically in 10 minute intervals	instrument units	optical_density
avg_OD720	optical density at 720 nm; averaged from 7 measurements taken automatically in 10 minute intervals	instrument units	optical_density
F0	instantaneous chlorophyll fluorescence (F0)	instrument units	fluor
cell_counts	cell concentrations obtained microscopically	cells/milliliter	cell_concentration
QY	the quantum yield: QY=Fv/Fm; where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity	dimensionless	Fv_Fm
Chla_repl_1	total chlorophyll a measured fluorometrically for replicate #1	microgram/liter	chl_a
Chla_repl_2	total chlorophyll a measured fluorometrically for replicate #2	microgram/liter	chl_a
POC_repl_1	Particulate organic carbon for replicate #1	microgram/liter	POC
POC_repl_2	Particulate organic carbon for replicate #2	microgram/liter	POC
PON_repl_1	Particulate orgainic nitrogen for replicate #1	microgram/liter	PON
PON_repl_2	Particulate orgainic nitrogen for replicate #2	microgram/liter	PON

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Dataset: Series 1B: light and temperature on growth follow-up expts