Dataset: 16S and nifH Sequence Data

Name: Metagenetic amplicon sequence data for multiple coral species from Curacao, Australia and Hawaii
Published: 2018-11-19
Keywords: oceans

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Cite This Dataset

Spatial Extent: N:21.4433 E:151.9148 S:-23.4423 W:-157.749

Project:

Nitrogen Fixing Prokaryotes in Corals: Is Nitrogen Fixation a Core Function of the Coral Microbiome? (NitroFixCorals)

Principal Investigator:

Michael P. Lesser (University of New Hampshire, UNH)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-11-19

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Metagenetic amplicon sequence data for multiple coral species from Curacao, Australia and Hawaii

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Description

These data can be found by accessing the NCBI Sequence Read Archive BioProject ID: PRJNA498285 (http://www.ncbi.nlm.nih.gov/bioproject/498285, last accessed 2018-11-14).

Metagenetic amplicon sequence FASTA file for multiple coral species from Heron Island, Great Barrier Reef, Australia (23.4423° S, 151.9148° E), Hawaii (21.4433° N, 157.7490° W), and Curacao (12.1696° N, 68.9900° W).

Methods & Sampling

Samples of corals were collected on shallow reefs in Australia, Hawaii and Curacao and preserved in DNA /RNA preservation buffer, Genomic DNA and RNA were extracted and sequenced. Sequences were assembled and annotated as required for each dataset.

Data Processing Description

fastq base calling and quality control

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1437054

Deployments

None available yet

Instruments

Automated DNA Sequencer

Supplied Name: Illumina MySeq

Supplied Description:

Genomic DNA and RNA were extracted and sequenced using Illumina MySeq

Instrument Type

Generic Name: Automated DNA Sequencer

Acronym: Automated Sequencer

Generic Description:

General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.

Parameters

None available yet

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Dataset: 16S and nifH Sequence Data