Dataset: trial_b
Data Citation:
Granger, J., Bourbonnais, A., Wilson, S. (2019) Trial B test of the dissolution method for estimates of the 15N2 atom% of incubations. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-02 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.778158.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.1575/1912/bco-dmo.778158.1
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Principal Investigator:
Julie Granger (University of Connecticut, UConn)
Co-Principal Investigator:
Annie Bourbonnais (University of Massachusetts Dartmouth, UMass Dartmouth)
Samuel Wilson (University of Hawai'i, UH)
BCO-DMO Data Manager:
Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2019-10-02
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Trial B test of the dissolution method for estimates of the 15N2 atom% of incubations
Abstract:
The “dissolution” method to measure N2 fixation rates with 15N2 gas tracer involves the preparation of 15N2-enriched water that is then added to each incubation bottle. Investigators typically measure the 15N2 atom% of the 15N2-enriched inoculum by MIMS, and extrapolate the 15N2 atom% in the incubations based on the inoculum value. Here, we demonstrate that such extrapolation yields inaccurate estimates of the 15N2 atom% of incubations. The latter should be measured directly.