Dataset: HPLC analysis

Name: High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico
Published: 2020-02-03
Keywords: phytoplankton pigments, HPLC, Gulf of Mexico, Mississippi River, phytoplankton community, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.789061.1

Spatial Extent: N:29.4883 E:-90.2333 S:28.375 W:-93.4167

Temporal Extent: 2017-09-18 - 2017-10-10

Project:

Collaborative Research: A RAPID response to Hurricane Harvey's impacts on coastal carbon cycle, metabolic balance and ocean acidification (HarveyCarbonCycle)

Principal Investigator:

Steven Lohrenz (University of Massachusetts Dartmouth, UMass Dartmouth)

Co-Principal Investigator:

Wei-Jun Cai (University of Delaware)

Sumit Chakraborty (Mote Marine Laboratory, Mote)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-02-03

Restricted:

Validated:

Yes

Current State:

Final no updates expected

High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico

Abstract:

High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2020-02-03	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.789061.1	2020-02-07

Description

High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico.

These data have been published in NASA SeaBASS Data Archive:

Methods & Sampling

Seawater samples for pigment analyses were immediately filtered (2 to 5 l volume) onto Whatman 47 mm GF/F filters under low vacuum (<0.5 atm). The filters were blotted dry, and stored in 2 ml cryotubes (Fisher) in liquid nitrogen until analysis. Analyses were performed within 6 months to a year of sampling.

Extraction procedures and HPLC analytical methods follow those described in Thomas (2012) and HPLC_Method_Summary.pdf (see related files and publications).

Data Processing Description

HPLC samples were analyzed during August-September 2018 at the Horn Point Analytical Laboratory by Meg Maddox (mmaddox@umces.edu). Values of -8888 indicate concentrations that were below detection levels. Values of -9999 indicate missing data. Coefficient of variation (replicate filter precision) for all NSF_Gulf_Rapid_HPLC samples was TChl a = 3.09%; Ppig = 4.28%.

The HPLC was controlled by Agilent Chemstation software.

Data Files

File
hplc_analysis.csv (Comma Separated Values (.csv), 28.29 KB) MD5:8563c655689125a464a0a82f3fedb25c Primary data file for dataset ID 789061

Supplemental Files

File
HPLC Method Summary filename: HPLC_method_summary.pdf (Portable Document Format (.pdf), 52.99 KB) MD5:89c2c743188087f66f7c866cb7ee00f5 High Performance Liquid Chromatography (HPLC) Method Summary by Crystal Thomas

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1760660
NSF Division of Ocean Sciences	OCE-1760509

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AC18-12	AC18_12_Roberts GOM_Discrete_1709 GOM_Nutrients_1709	2017-09-17	R/V Acadiana	John Lehrter (Chief Scientist)	data info
PE18-11	GOM_CTD_1710 GOM_Discrete_1710 GOM_Discrete_1710	2017-09-28	R/V Pelican	Baoshan Chen (Chief Scientist)	data info

Instruments

High-Performance Liquid Chromatograph

Supplied Name: Agilent RR1200 HPLC

Supplied Description:

Agilent RR1200 HPLC controlled bu Agilent Chemstation software

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Hpl_id	Lab Sample ID	unitless	sample
Sample	Cruise Sample ID	unitless	sample
Station	Station	unitless	station
Volfilt	Volume Filtered	liter (L)	vol_filt
Depth	Sample Depth	meter (m)	depth
Date	Date (yyyymmdd) in UTC	unitless	date
Time	Time (hh:mm:ss)	unitless	time
Lat	Latitude (N)	decimal degrees	lat
Lon	Longitude (E)	decimal degrees	lon
Tot_Chl_a	DV_Chl_a + MV_Chl_a + Chlide_a + Chl_a_allom + Chl_a_prime	milligram per cubic meter (mg/m^3)	chl_a
Tot_Chl_b	DV_Chl_b + MV_Chl_b	milligram per cubic meter (mg/m^3)	chl_b
Tot_Chl_c	chl_c1 + chl_c2 (i.e.: chl_c1c2) + chl_c3	milligram per cubic meter (mg/m^3)	chl_c
alpha_beta_Car	Alpha (Beta;epsilon) + Beta (Beta;beta) Carotenes	milligram per cubic meter (mg/m^3)	carotene
But_fuco	19'-Butanoyloxyfucoxanthin	milligram per cubic meter (mg/m^3)	fucox
Hex_fuco	19'-Hexanoyloxyfucoxanthin	milligram per cubic meter (mg/m^3)	fucox
Allo	Alloxanthin	milligram per cubic meter (mg/m^3)	allox
Diadino	Diadinoxanthin	milligram per cubic meter (mg/m^3)	diadinox
Diato	Diatoxanthin	milligram per cubic meter (mg/m^3)	diatox
Fuco	Fucoxanthin	milligram per cubic meter (mg/m^3)	fucox
Perid	Peridin	milligram per cubic meter (mg/m^3)	peridinin
Zea	Zeaxanthin	milligram per cubic meter (mg/m^3)	zeax
MV_Chl_a	Monovinyl Chorophyll a	milligram per cubic meter (mg/m^3)	chl_a1
DV_Chl_a	Divinyl Chorophyll a	milligram per cubic meter (mg/m^3)	chl_a2
Chlide_a	Chlorophyllide a	milligram per cubic meter (mg/m^3)	chlide
MV_Chl_b	Monovinyl Chorophyll b	milligram per cubic meter (mg/m^3)	chl_b1
DV_Chl_b	Divinyl Chorophyll b	milligram per cubic meter (mg/m^3)	chl_b2
Chl_c1c2	Chlorophyll c1 + c2 + Mg 2;4 divinyl pheoporphyrin a5 monomethyl ester	milligram per cubic meter (mg/m^3)	chl_c1_c2
Chl_c3	Chlorophyll c3	milligram per cubic meter (mg/m^3)	chl_c3
Lut	Lutein	milligram per cubic meter (mg/m^3)	lutein
Neo	Neoxanthin	milligram per cubic meter (mg/m^3)	neox
Viola	Violaxanthin	milligram per cubic meter (mg/m^3)	violax
Phytin_a	Pheophytin a	milligram per cubic meter (mg/m^3)	p_phytin
Phide_a	Pheophorbide a	milligram per cubic meter (mg/m^3)	p_phorbide
Pras	Prasinoxanthin	milligram per cubic meter (mg/m^3)	prasinox
Gyro	Gyroxanthin	milligram per cubic meter (mg/m^3)	gyrox
TChl	Tot_Chl_a + Tot_Chl_b + Tot_Chl_c	milligram per cubic meter (mg/m^3)	chl_tot
PPC	Photoprotective carotenoids (Allo + Diadino + Diato + Zeo + alpha-beta-Car)	milligram per cubic meter (mg/m^3)	car_ppc
PSC	Photosynthetic carotenoids (But-fuco + Fuco + Hex-fuco + Perid)	milligram per cubic meter (mg/m^3)	car_psc
PSP	Photosynthetic pigments (PSC + Tchl)	milligram per cubic meter (mg/m^3)	chl_psp
Tcar	Total Carotenoids (PPC + PSC)	milligram per cubic meter (mg/m^3)	car_psc_psp
Tacc	Total accessory pigments (PPC + PSC + Tot_Chl_b + Tot_Chl_c)	milligram per cubic meter (mg/m^3)	pig_tot_acc
Tpg	Total pigment concentration	milligram per cubic meter (mg/m^3)	pig_tot
DP	Total diagnostic pigments (PSC + Allo + Zea + Tot_Chl_b)	milligram per cubic meter (mg/m^3)	pig_tot
Tacc_Tchla	Ratio of Tacc to Tot_Chl_a	unitless	pigacc_to_chla_tot
PSC_Tcar	Ratio of PSC to Tcar	unitless	pigpsc_to_cartot
PPC_Tcar	Ratio of PPC to Tcar	unitless	pigppc_to_cartot
TChl_Tcar	Ratio of Tchl to Tcar	unitless	chltot_to_cartot
PPC_Tpg	Ratio of PPC to Tpg	unitless	pigppc_to_pigtot
PSP_Tpg	Ratio of PSC to Tpg	unitless	no_bcodmo_term
TChla_Tpg	Ratio of Tot_chl_a to Tpg	unitless	chla_tot_to_pigtot
Cruise	Cruise ID (AC18_12_Roberts or PE18-11)	unitless	cruise_id
ISO_DateTime_UTC	Date and time (UTC) in ISO format (yyyy-mm-ddThh:mm:ss)	unitless	ISO_DateTime_UTC

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