Dataset: Cryopeg Holographic Images

Name: Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017
Published: 2020-07-01
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.817454.1

Spatial Extent: N:71.473 E:-156.5049 S:71.2944 W:-156.7294

Temporal Extent: 2017-05-06 - 2017-05-10

Project:

Understanding How Virus Infection Affects Gene Flow and Microbial Evolution in Extreme Polar Environments (Arctic Subzero Brines)

Program:

Marine Microbiology Initiative (MMI)

Principal Investigator:

Jody W. Deming (University of Washington, UW)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-07-01

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017

Abstract:

Unprocessed (raw) holographic data of cryopeg and sea ice brines viewed using a digital holographic microscope at sub-micron resolution. Samples collected from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice near Utqiagvik in May 2017.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2020-07-01	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.817454.1	2020-07-16

Description

Brine samples were collected from both sea ice and cryopeg near Utqiaġvik, Alaska, USA. Snow and ice thickness along with sackhole core depth information are available for sea ice samples. Bacterial and viral abundances along with temperature, pH, salinity, inorganic nutrients, organic nutrients, EPS, and water isotopes were measured for select samples.

Methods & Sampling

Sackhole and cryopeg brines were collected according to Cooper et al., 2019 FEMS Environmental Microbiology and inserted into the holographic microscope using a syringe as described in Lindensmith et al., 2016 PLOS One.

Experiments were completed at the sample site or in the field-laboratory using a chemotactic chamber as described in Lindensmith, et al., 2016 PLOS One. Chemical gradients of serine (prepared to 1 molar concentration in self-same salinity solution) or gradients of salinity (established from sample salinity to seawater) were used to stimulate taxis across the chamber, or the a heating block was used to create temperature gradients in a cold room. Motility was observed in the microscope sub-sampling over periods of seconds or hours, according to timestamp data (available in the hologram folder).

Data Processing Description

BCO-DMO Processing Notes:
- packaged all images into tar gzipped file.

Data Files

File

Cryopeg Holographic Microscopy Images
filename: images.tar.gz

(GZIP (.gz), 29.82 GB)
MD5:64834f891e9de0228564641b4546f63c

Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution. Below is a list of the directories contained in the package with a brief description of the conditions.

05.06 16-12	Sea ice sackhole brine in situ,  (115 ppt)

05.07 18-54	Cryopeg brine, -6C, diluted 1:100 in seawater

05.07 19-11	Cryopeg brine, -6C, diluted 1:100 in seawater

05.07 22-15	Sackhole brine 2, +5c, chemotaxis experiment toward 1 M serine

05.08 01-06	Permafrost tunnel brine, room temperature

05.08 01-14	Permafrost tunnel brine, room temperature

05.08 01-18	Permafrost tunnel brine, room temperature

05.08 22-46	Cryopeg brine, -6C

05.08 23-00	Cryopeg brine, 5C

05.09 01-23	Cryopeg brine, +4C

05.09 02-53	Cryopeg brine, -1C, halotaxis experiment toward seawater salinity

05.09 03-10	Cryopeg brine, -1C, thermotaxis experiment toward 32C

05.09 03-12	Cryopeg brine, -1C, thermotaxis experiment toward 16C

05.09 03-15	Cryopeg brine, -1C, thermotaxis experiment toward 8C

05.09 12-39	Cryopeg brine, -1C, chemotaxis experiment toward 1 M serine

05.10 01-39	Sackhole 2 brine, -1C, haloxtaxis experiment toward seawater salinity

More Information about this dataset

Funding Sources

Funding Source	Award Number
Gordon and Betty Moore Foundation: Marine Microbiology Initiative	GBMF5488

Deployments

None available yet

Instruments

Digital inline holographic microscope

Supplied Name: holographic microscope

Supplied Description:

Instrument Type

Generic Name: Digital inline holographic microscope

Acronym: DIHM

Generic Description:

A Digital Inline Holographic Microscope (DIHM) uses coherent (laser) light and a digital camera to image objects with micrometer scale resolution. A portion of the light scattered by illuminated objects interferes with incident light in a predictable manner. The resulting interference patterns projected onto a two-dimensional plane (i.e. digital camera sensor) are recorded as holograms. These digital holograms are then numerically reconstructed to produce an in-focus image at a given distance from the recording plane. A relatively large illuminated volume (>100 mL) can be reconstructed in this manner to produce a single image with an extended depth of field.

Parameters

None available yet

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Database

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Dataset: Cryopeg Holographic Images