Dataset: Proportion infected polyps – Fall 2018 experiment

Name: Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.
Published: 2021-11-16
Keywords: Symbiosis, Symbiodiniaceae, Octocorals, thermal tolerance, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.855162.1

Spatial Extent: N:24.7525 E:-81.754583 S:24.7525 W:-81.754583

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

Co-Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-16

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.

Abstract:

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-11-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.855162.1	2021-11-16

Methods & Sampling

Larvae were collected from Antillogorgia bipinnata colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL. Antillogorgia bipinnata colonies were initially collected from Tennessee Reef (N 24° 45.150' W 81° 45.275').

Breviolum antillogorgium cultures, representing five genotypes, were used to infect polyps reared from larvae collected from A. bipinnata. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]). Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. Containers with polyps were inoculated on Day 1 and Day 5. Starting on Day 32, containers were inoculated three times a week with water changes. Polyp location in the containers was mapped and infection status for each individual polyp was recorded every 3-4 days through visual observation as indicated by a light brown coloration in the polyp. At the end of the experiment (Day 69) infection status was based on cell counts.

Data Processing Description

Proportion and percentage of infected polyps was calculated at three timepoints and was determined based on the number of polyps alive at that time.

Infection status of the polyps was assessed visually using a Leica dissecting microscope approximately every 3 days from Day 1 – Day 67 and by cell counts on Day 69.

BCO-DMO processing notes:

Adjusted parameter names to comply with database requirements

Data Files

File
infected_polyps.csv (Comma Separated Values (.csv), 5.46 KB) MD5:9712186b9aba01c072cd7df46c9ab535 Primary data file for dataset ID 855162

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559286

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Leica dissecting microscope

Supplied Description:

Infection status of the polyps was assessed visually using a Leica dissecting microscope approximately every 3 days from Day 1 – Day 67 and by cell counts on Day 69. See BCO-DMO dataset “Cell counts in polyps – Fall 2018 experiment”

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Genotype	Genotype of culture (based on 5 microsatellite loci) used to inoculate the polyps,	Unitless	sample_descrip
Temperature	Temperature at which the polyp was reared	Degrees Celsius (°C)	temperature
Container	Identification of container replicate	Unitless	sample_descrip
Day	Day indicates the timepoint, post-inoculation, at which proportion of infected polyps was determined.	Unitless	sample_descrip
Proportion_infected	Proportion infected polyps at that timepoint and was determined based on the number of polyps alive at that time. nd indicates that there were no surviving polyps at that time point. No polyps Alive	Unitless	sample_descrip
Percent_infected	Percentage infected polyps at that timepoint and was determined based on the number of polyps alive at that time. nd indicates that there were no surviving polyps at that time point. No polyps Alive	Unitless	sample_descrip

Status message

Database

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Dataset: Proportion infected polyps – Fall 2018 experiment