Dataset: BF In Situ Experiments

Name: Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019
Published: 2021-11-02
Keywords: oceans

View Data: Data not available yet

Cite This Dataset

Spatial Extent: N:31.7635 E:-79.8421 S:31.0175 W:-80.7965

Temporal Extent: 2019-03-30 - 2019-04-10

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-02

Restricted:

Yes

Release Date:

2022-07-26

Validated:

Current State:

Data not available

Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019

Abstract:

Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.

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Methods & Sampling

Sampling and analytical procedures:

Seawater samples for bioavailability factor determinations were collected from Niskin bottles (12 L), at station 1 (two measurements) and 3 (one measurement) along a transect from coastal Georgia to offshore waters. The bioavailability factor was calculated based on changes in PO43- turnover time. The bioavailability factor (BF) is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where TE reflects PO43- turnover time in the DOP amended treatment, TN the PO43- turnover time in the control (no additions) and TP the PO43- turnover time in the treatment amended with Pi. In this study, treatments were amended with polyphosphate (Polyp), adenosine triphosphate (ATP), adenosine monophosphate (AMP) or methyl phosphonate (Mepn).

BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.

Instruments: Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.

Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Problem report: BF in station 1 (first determination) were determined in duplicates instead of triplicate in station 1 (second determination) and station 3. Therefore, gaps are filled with NaN in the .mat files.

Data Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737083
NSF Division of Ocean Sciences	OCE-2001212
NSF Division of Ocean Sciences	OCE-1736967
NSF Division of Ocean Sciences	OCE-1948042

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
SAV-19-02	Zephyr Zooming in on Enzymatic PhosphoHYdrolysis Reactions	2019-03-30	R/V Savannah	Julia Diaz (Chief Scientist)	data info

Instruments

Liquid Scintillation Counter

Supplied Name:

Supplied Description:

Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample.

Niskin bottle

Supplied Name:

Supplied Description:

Sampling was performed using Niskin bottles (12 L) mounted on a rosette.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

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Dataset: BF In Situ Experiments