Dataset: Acer Fecundity Polpys per Area

Name: Assessment of polyps per area of Acropora cervicornis genotypes sampled July 2020 for fecundity analysis at Mote Marine Laboratory
Published: 2022-03-16
Keywords: Coral, Fecundity, polyps, biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.868308.1

Spatial Extent: N:24.56257 E:-81.4001 S:24.4578 W:-81.8859

Temporal Extent: 2020-07-03

Project:

CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys (Resilient Acerv)

Principal Investigator:

Erinn M. Muller (Mote Marine Laboratory, Mote)

Co-Principal Investigator:

Hanna Koch (Mote Marine Laboratory, Mote)

Scientist:

Erich Bartels (Mote Marine Laboratory, Mote)

Student:

Yuen Azu (Mote Marine Laboratory, Mote)

Contact:

Hanna Koch (Mote Marine Laboratory, Mote)

BCO-DMO Data Manager:

Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-03-16

Restricted:

Release Date:

2022-12-31

Validated:

Yes

Current State:

Final no updates expected

Assessment of polyps per area of Acropora cervicornis genotypes sampled July 2020 for fecundity analysis at Mote Marine Laboratory

Abstract:

Primary fecundity was assessed for Acropora cervicornis corals with known disease susceptibility. This dataset presents information on the number of polyps per area from linear branches of five colonies with 12 genets held in Mote Marine Lab’s spawning nurseries.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2022-03-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.868308.1	2022-03-24

Description

This dataset is part of a larger study of Acropora cervicornis (staghorn) corals studied at Mote Marine Lab’s Elizabeth Moore International Center for Coral Reef Research and Restoration. The different analyses are listed here, and links to other data from this study can be found in the Related Datasets section below.

Analyses undertaken include:

Total Population (Colony size dataset)
Morphometric Assessment (Colony size dataset)
Primary Fecundity Analysis (Population subset of Colony size dataset; and this Polyps dataset)
Dissections (Oocyte number dataset, Oocyte size dataset)
Secondary Fecundity Analysis (Gamete bundle dataset)

See Related Datasets section below for links to above mentioned datasets.

Methods & Sampling

Sampling of Acropora cervicornis coral genotypes took place at Mote Marine Lab's spawning nurseries at Sand Key (24.45782, -81.88595) and Looe Key (24.56257, -81.40009).

Sampling for Primary Fecundity Analysis
On July 3, 2020, from 5 colonies of every genet, 3 linear branches (~10 cm in length) were sampled using bone cutters from the central portion of each colony (N=180 branches). Using a ruler, the number of polyps per 1 cm^2 was recorded from every branch near the base of the fragment. The top ~2 cm (sterile zone) of every branch was removed before placing into a 50 mL falcon tube with 10% formalin to fix tissues. After 2 days, the formalin solutions were replaced with a 5% HCl solution, with every branch and tube triple rinsed with DI water in between to remove excess formalin. After 3 days, the 5% HCl solution in every tube was replaced with 10% HCl and subsequently refreshed every 2-3 days until branches were completely decalcified. Once decalcified, samples were triple rinsed in DI water and returned to their tubes with 70% EtOH for storage until dissection.

Dissections
Under a dissecting microscope, every fragment was dissected using a scalpel and forceps to haphazardly select 5 polyps per fragment (N = 900 polyps) to count the total number of oocytes within each polyp (Oocyte Number dataset, https://www.bco-dmo.org/dataset/867314). From those, 5 oocytes were randomly selected to measure their size (Oocyte Size Dataset: https://www.bco-dmo.org/dataset/843067)

Data Processing Description

Data processing:
Data were compiled into Excel (Microsoft Office) and analyzed using R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out". Nonparametric statistical and correlation analyses were conducted.

BCO-DMO Processing:
- separated Latitude and Longitude into separate columns
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
polyps.csv (Comma Separated Values (.csv), 9.03 KB) MD5:f12908cd2b4ac9bb04fa8c51bf951709 Primary data file for dataset ID 868308

Supplemental Files

File
Disease_Susceptibility_Table filename: Disease_Susceptibility_Table.pdf (Portable Document Format (.pdf), 64.24 KB) MD5:b155930985bd0c9e954a1f0eeacc78a1 Acropora cervicornis genotypes and susceptibility to white-band disease

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1452538

Deployments

None available yet

Instruments

bone cutter

Supplied Name: Calcutta metal pliers

Supplied Description:

Each ramet was cut from the donor colony using metal pliers (Calcutta bone cutters).

Instrument Type

Generic Name: bone cutter

Generic Description:

A bone cutter is a surgical instrument used to cut bones or coral fragments.

ruler

Supplied Description:

the dimensions of length, width, and height (L x W x H) in centimeters were measured.

Instrument Type

Generic Name: ruler

Generic Description:

A device used for measuring or for drawing straight lines, consisting of an elongated piece of rigid or semi-rigid material marked with units for measurement. Device that allows one or more physical dimensions of a sample or specimen to be determined by visible comparison against marked graduations in units of measurement of dimension length.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Location	Location of coral sampling site	unitless	site
Latitude	Latitude of spawning nursery	decimal degrees	lat
Longitude	Longitude of spawning nursery	decimal degrees	lon
Date_measured	Date when measurements were taken (local time) Format: %Y-%m-%d	unitless	date
Genotype	Mote Marine Lab genet that was sampled (1, 3, 7, 13, 31, 34, 41, 44, 47, 50, 62, 63)	unitless	sample_type
Phenotype	Phenotype that was sampled (S = white band disease susceptible, R = white band disease resistant)	unitless	sample_type
Replicate_Colony	Which of the five replicate colonies of the genet was sampled for analysis	unitless	replicate
Replicate_Fragment	Which of the three replicate fragments per replicate colony was sampled for analysis	unitless	replicate
Number_Polyps	Number of polyps measured within a one square centimeter area of a coral fragment	polyps per square centimeter	no_bcodmo_term

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Database

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Dataset: Acer Fecundity Polpys per Area