Dataset: Breviolum Symbiont Microsatellite Genotypes in Orbicella faveolata Recruits

Name: Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project)
Published: 2023-03-07
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.887265.1

Spatial Extent: N:25.1167 E:-80.2833 S:24.5449 W:-81.4094

Temporal Extent: 2009 - 2011

Project:

Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)

Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

Co-Principal Investigator:

Margaret W. Miller (NOAA Southeast Fisheries Science Center, NOAA SEFSC)

David Sheets (Canisius College)

Student:

Noel J. Leigh (State University of New York at Buffalo, SUNY Buffalo)

Shelby E. McIlroy (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-03-07

Restricted:

Release Date:

2022-11-09

Validated:

Yes

Current State:

Final no updates expected

Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project)

Abstract:

Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits reared in a field-based lab and then outplanted to reefs in the Florida Keys.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-03-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.887265.1	2023-05-24

Methods & Sampling

Sampling and Analytical Methodology:

Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014). Larvae were reared at a shore-based lab and settled onto terracotta tiles. Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992). Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009).

Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992). Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

Locations in the Florida Keys with Abbreviations

Alligator (AR)
Coral Garden (CG)
Cheeca Rocks (CR)
East Turtle (ET)
Grecian Rocks (GR)
Looe Key (LK)
Sand Island (SI)
Tennessee (TR)

Data Processing Description

Processing Notes from Researcher:

For parameters B7Sym34, B7Sym36, CA6.38 (CA_6_38), Assigned_Genotype, and CP_type, NA indicates "no amp - did not amplify after multiple attempts," and ND indicates "not determined because one or more loci did not amplify."

BCO-DMO Processing Notes:

Lat and Long split into separate columns, converted to decimal degrees, and rounded to 6 decimal places.

Data Files

File
microsatellite_genotypes_recruits-1.csv (Comma Separated Values (.csv), 7.37 KB) MD5:b13634855201c53179bda055c0b8c5c0 File processed with laminar pipeline "887265_v1_breviolum_symbiont_microsatellite_genotypes" at path 887265/1/data/microsatellite_genotypes_recruits-1.csv

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0926822

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_ID	Identification of Orbicella faveolata recruit sampled.	unitless	sample
Year	Year in which sample was collected.	unitless	year
Site	Reef where egg-sperm bundles were collected from O. faveolata adult colonies; site of parental colonies. AR = Alligator; CG = Coral Garden; CR = Cheeca Rocks; ET = East Turtle; GR = Grecian Rocks; LK = Looe Key; SI = Sand Island; TR = Tennessee.	unitless	site
Colony_Number	Identification number for the related colony.	unitless	site
Location_in_colony	Location within the colony where the sample as taken; Top-T, Middle-M, Bottom-B.	unitless	site
Depth_m	Depth where sample was taken.	meters (m)	depth
Latitude_decimal_degrees	Latitude of reef where the sample was taken in decimal degrees. A positive value indicates North.	decimal degrees	lat
Longitude_decimal_degrees	Longitude of reef where the sample was taken in decimal degrees. A negative value indicates West.	decimal degrees	lon
B7Sym34	Fragment size of the B7Sym34 microsatellite allele.	basepair	no_bcodmo_term
B7Sym36	Fragment size of the B7Sym36 microsatellite allele.	basepair	no_bcodmo_term
CA_6_38	Fragment size of the CA6.38 microsatellite allele.	basepair	no_bcodmo_term
Assigned_Genotype	Genotype assigned based on the combination of microsatellite alleles at the three loci. Numbering is arbitrary. ND indicates that a value was Not Determined because one or more loci did not amplify.	unitless	no_bcodmo_term

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Database

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Dataset: Breviolum Symbiont Microsatellite Genotypes in Orbicella faveolata Recruits